MG1655
W3110
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Gene Report : sapA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion 20 (LD)  ,  OCR70 (D)  ,  OCR70,L90-5 (LD 20-1_P/O) (D)  ,  OCR70,L90-6 (LD 20-1_P/O & 20-2TC) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  sapA
Related strains
     Strains Search Search strains by gene name:  sapA   Search strains by all related name:  sapA b1294 ECK1289 f547 JW1287

General information  (Go to Linear View:)
 Gene Name sapA  
 Alternative name b1294,ECK1289,f547,JW1287  
 Location, Length 1,353,491 - 1,355,134 (  -  ) ;   29.17 min ; 1644 (bp) ,   547 (aa) Go to Linear View
 Product antimicrobial peptide transport ABC transporter periplasmic binding protein  
 Operon Name sapABCDF  
 Note homolog of Salmonella peptide transport periplasmic protein; periplasmic-binding component of ABC superfamily; GO_component: GO:0042597 - periplasmic space; GO_process: GO:0009063 - cellular amino acid catabolic process  
 Function putative transport; Not classified  
 Gene Ontology GO:0005215 ; transporter activity ( sapA )
GO:0006810 ; transport ( sapA )
GO:0006857 ; oligopeptide transport ( sapA )
GO:0015031 ; protein transport ( sapA )
GO:0015198 ; oligopeptide transporter activity ( sapA )
GO:0042597 ; periplasmic space ( sapA )
 PID 1787551  
 EC number
  (KEGG Pathway)   		 
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) SAPA_ECOLI ( Q47622 ; P77358 )
    -  Protein name Peptide transport periplasmic protein sapA precursor.  
    -  Synonyms  
    -  Gene name Name=sapA; OrderedLocusNames=b1294;  

 Linear View (Whole Mode)
View Location
   1345.0  –  1370.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP sapA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Dipeptide Binding Protein Complex With Glycyl-L-Leucine  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Peptide transport periplasmic protein sapA precursor  
         PSI-BLAST Peptide transport periplasmic protein sapA precursor
    nr     (database updated : 2007.02.20 )
         BLAST predicted antimicrobial peptide transporter subunit [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    SBP_BACTERIAL_5     

Other Cross-Reference
    COG COG4166E 
    EcoCyc sapA 

MMBR References
GenBank submission U08190. 1994

Bergler, H., et al.
EMBO J. 1993;12:4053-4062
Molecular genetic analysis of a locus required for resistance to antimicrobial peptides in Salmonella typhimurium.
Parra-Lopez, C., M. T. Baer, and E. A. Groisman.
Personal communication.

Wu, H. C., and K. Rudd.

Sequences
Amino acid
FASTA format 
0001 MRQVLSSLLV IAGLVSGQAI AAPESPPHAD IRDSGFVYCV SGQVNTFNPS KASSGLIVDT LAAQFYDRLL 
0071 DVDPYTYRLM PELAESWEVL DNGATYRFHL RRDVPFQKTD WFTPTRKMNA DDVVFTFQRI FDRNNPWHNV 
0141 NGSNFPYFDS LQFADNVKSV RKLDNHTVEF RLAQPDASFL WHLATHYASV MSAEYARKLE KEDRQEQLDR 
0211 QPVGTGPYQL SEYRAGQFIR LQRHDDFWRG KPLMPQVVVD LGSGGTGRLS KLLTGECDVL AWPAASQLSI 
0281 LRDDPRLRLT LRPGMNVAYL AFNTAKPPLN NPAVRHALAL AINNQRLMQS IYYGTAETAA SILPRASWAY 
0351 DNEAKITEYN PAKSREQLKS LGLENLTLKL WVPTRSQAWN PSPLKTAELI QADMAQVGVK VVIVPVEGRF 
0421 QEARLMDMSH DLTLSGWATD SNDPDSFFRP LLSCAAIHSQ TNLAHWCDPK FDSVLRKALS SQQLAARIEA 
0491 YDEAQSILAQ ELPILPLASS LRLQAYRYDI KGLVLSPFGN ASFAGVYREK QDEVKKP
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             aaccacacgg ccctcattac gacccctgaa 
-070 cacaaacggt aatggcaaat gtcattaaat attagtaaga tagcctcttt acataaaatc ccttaatatt 
0001 atgcgccagg tattatcgtc tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg 
0071 aatctccccc gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt 
0141 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga tcgactgctg 
0211 gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg ggaagtactc gacaacggcg 
0281 cgacctatcg cttccacctg cgtcgcgatg ttccgtttca aaaaaccgac tggtttactc ccactcgtaa 
0351 aatgaatgcc gacgatgtgg tgtttacctt ccagcgaatt tttgaccgca acaacccgtg gcataacgtc 
0421 aacggcagca acttccccta cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg 
0491 ataatcatac cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta 
0561 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca actcgaccgt 
0631 caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca atttattcgc ctacaacgtc 
0701 atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt agtggtggat ttaggctccg gcggcaccgg 
0771 acgtctgtcg aaactcctga ccggggaatg cgacgttctg gcctggcctg ctgccagcca gctatccatt 
0841 ttgcgtgacg acccgcgctt gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca 
0911 ccgccaaacc gccgctaaat aatcccgctg tccgccatgc gctggcactg gcgattaata accagcgcct 
0981 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc gtgggcctat 
1051 gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca gttgaagtcg ttggggctgg 
1121 aaaatttaac gctgaaactg tgggtgccca cacgttcgca ggcgtggaac cccagtccac tgaaaactgc 
1191 cgaactgatt caggcggata tggcgcaggt tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt 
1261 caggaggcgc ggttgatgga tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc 
1331 cggacagttt cttccgtccg ttactgagct gcgcggcaat tcattcgcag accaacctcg cccactggtg 
1401 cgatccgaaa ttcgacagcg tattgcgtaa ggcgctctct tcgcagcagc tggcggcgcg tattgaagcc 
1471 tatgacgaag cgcagagtat tctggcgcag gaattgccca ttttgccgct ggcgtcgtca ttgcgtttgc 
1541 aggcctatcg gtacgatatc aaaggtctgg tacttagccc gtttggtaac gcctcctttg ctggggtgta 
1611 tcgcgagaaa caggatgagg tgaaaaaacc atgattatct tcaccttacg ccgcattttg ttattgattg 
1681 tcaccttgtt cctgctgacc ttcgttggct tcagcttgag ctatttcacc cctcatgcgc cgtt