MG1655
W3110
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Gene Report : narZ
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion 20 (LD)  ,  DE(PLK1608)  ,  OCR70,L90-5 (LD 20-1_P/O) (D)  ,  OCR70,L90-6 (LD 20-1_P/O & 20-2TC) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  narZ
Related strains
     Strains Search Search strains by gene name:  narZ   Search strains by all related name:  narZ b1468 chlZ ECK1462 JW1463

General information  (Go to Linear View:)
 Gene Name narZ  
 Alternative name b1468,chlZ,ECK1462,JW1463  
 Location, Length 1,536,874 - 1,540,614 (  -  ) ;   33.12 min ; 3741 (bp) ,   1246 (aa) Go to Linear View
 Product nitrate reductase 2 (NRZ), alpha subunit  
 Operon Name narZYWV  
 Note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009061 - anaerobic respiration  
 Function enzyme; Energy metabolism, carbon: Anaerobic respiration  
 Gene Ontology GO:0005488 ; binding ( narZ )
GO:0005506 ; iron ion binding ( narZ )
GO:0005515 ; protein binding ( narZ )
GO:0005886 ; plasma membrane ( narZ )
GO:0006118 ; electron transport ( narZ )
GO:0006810 ; transport ( narZ )
GO:0008940 ; nitrate reductase activity ( narZ )
GO:0009325 ; nitrate reductase complex ( narZ )
GO:0016020 ; membrane ( narZ )
GO:0016491 ; oxidoreductase activity ( narZ )
GO:0030151 ; molybdenum ion binding ( narZ )
GO:0042126 ; nitrate metabolic process ( narZ )
GO:0042128 ; nitrate assimilation ( narZ )
GO:0046872 ; metal ion binding ( narZ )
GO:0051536 ; iron-sulfur cluster binding ( narZ )
GO:0051539 ; 4 iron, 4 sulfur cluster binding ( narZ )
 PID 1787741  
 EC number
  (KEGG Pathway)   		1.7.99.4  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) NARZ_ECOLI ( P19319 ; P78063; P78154; P78155; P78156 )
    -  Protein name Respiratory nitrate reductase 2 alpha chain  
    -  Synonyms EC 1.7.99.4  
    -  Gene name Name=narZ; OrderedLocusNames=b1468;  

 Linear View (Whole Mode)
View Location
   1525.0  –  1550.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP narZ (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Nitrate Reductase A, Narghi, From Escherichia Coli  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Respiratory nitrate reductase 2 alpha chain  
         PSI-BLAST Respiratory nitrate reductase 2 alpha chain
    nr     (database updated : 2007.02.20 )
         BLAST nitrate reductase 2 (NRZ), alpha subunit [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    PROKAR_LIPOPROTEIN    MOLYBDOPTERIN_PROK_2    MOLYBDOPTERIN_PROK_1    MOLYBDOPTERIN_PROK_3     

Other Cross-Reference
    COG COG5013C 
    EcoCyc narZ 

MMBR References
Mol Gen Genet. 1990;222(1):104-11.
Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon.
Blasco F, Iobbi C, Ratouchniak J, Bonnefoy V, Chippaux M. ( 2233673 )
Mol Microbiol. 1992;6(2):221-30.
Involvement of the narJ or narW gene product in the formation of active nitrate reductase in Escherichia coli.
Blasco F, Pommier J, Augier V, Chippaux M, Giordano G. ( 1545706 )
Mol Microbiol. 1987;1(2):143-50.
Presence in the 'silent' terminus region of the Escherichia coli K12 chromosome of cryptic gene(s) encoding a new nitrate reductase.
Bonnefoy V, Burini JF, Giordano G, Pascal MC, Chippaux M. ( 3329696 )

Sequences
Amino acid
FASTA format 
0001 MSKLLDRFRY FKQKGETFAD GHGQVMHSNR DWEDSYRQRW QFDKIVRSTH GVNCTGSCSW KIYVKNGLVT 
0071 WEIQQTDYPR TRPDLPNHEP RGCPRGASYS WYLYSANRLK YPLIRKRLIE LWREALKQHS DPVLAWASIM 
0141 NDPQKCLSYK QVRGRGGFIR SNWQELNQLI AAANVWTIKT YGPDRVAGFS PIPAMSMVSY AAGTRYLSLL 
0211 GGTCLSFYDW YCDLPPASPM TWGEQTDVPE SADWYNSSYI IAWGSNVPQT RTPDAHFFTE VRYKGTKTIA 
0281 ITPDYSEVAK LCDQWLAPKQ GTDSALAMAM GHVILKEFHL DNPSDYFINY CRRYSDMPML VMLEPRDDGS 
0351 YVPGRMIRAS DLVDGLGESN NPQWKTVAVN TAGELVVPNG SIGFRWGEKG KWNLESIAAG TETELSLTLL 
0421 GQHDAVAGVA FPYFGGIENP HFRSVKHNPV LVRQLPVKNL TLVDGNTCPV VSVYDLVLAN YGLDRGLEDE 
0491 NSAKDYAEIK PYTPAWGEQI TGVPRQYIET IAREFADTAH KTHGRSMIIL GAGVNHWYHM DMNYRGMINM 
0561 LIFCGCVGQS GGGWAHYVGQ EKLRPQTGWL PLAFALDWNR PPRQMNSTSF FYNHSSQWRY EKVSAQELLS 
0631 PLADASKYSG HLIDFNVRAE RMGWLPSAPQ LGRNPLGIKA EADKAGLSPT EFTAQALKSG DLRMACEQPD 
0701 SSSNHPRNLF VWRSNLLGSS GKGHEYMQKY LLGTESGIQG EELGASDGIK PEEVEWQTAA IEGKLDLLVT 
0771 LDFRMSSTCL FSDIVLPTAT WYEKDDMNTS DMHPFIHPLS AAVDPAWESR SDWEIYKGIA KAFSQVCVGH 
0841 LGKETDVVLQ PLLHDSPAEL SQPCEVLDWR KGECDLIPGK TAPNIVAVER DYPATYERFT SLGPLMDKLG 
0911 NGGKGISWNT QDEIDFLGKL NYTKRDGPAQ GRPLIDTAID ASEVILALAP ETNGHVAVKA WQALGEITGR 
0981 EHTHLALHKE DEKIRFRDIQ AQPRKIISSP TWSGLESDHV SYNAGYTNVH ELIPWRTLSG RQQLYQDHPW 
1051 MRAFGESLVA YRPPIDTRSV SEMRQIPPNG FPEKALNFLT PHQKWGIHST YSENLLMLTL SRGGPIVWIS 
1121 ETDARELTIV DNDWVEVFNA NGALTARAVV SQRVPPGMTM MYHAQERIMN IPGSEVTGMR GGIHNSVTRV 
1191 CPKPTHMIGG YAQLAWGFNY YGTVGSNRDE FIMIRKMKNV NWLDDEGRDQ VQEAKK
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             gaagttcagc caaaaataag agatcgatgc 
-070 ctgatgtaca accgtgtcgc atccgacaag gcatcagatc ccaattcatt cctggagcag gagtcatgtc 
0001 atgagtaaac ttttggatcg ctttcgctac ttcaaacaaa agggcgaaac ctttgccgat ggtcacggac 
0071 aggtgatgca tagcaaccgc gactgggagg acagctatcg ccagcgttgg cagttcgaca aaatcgtgcg 
0141 ttccacccac ggtgttaact gtacaggctc ctgtagctgg aaaatctacg ttaaaaatgg tctggtgacc 
0211 tgggaaatcc aacagaccga ctacccgcgc actcgccctg acctgcccaa tcatgaacct cgcggctgcc 
0281 cgcgtggcgc aagttactcc tggtatcttt acagcgctaa ccgcctgaaa tacccgctca ttcgtaaacg 
0351 actgattgaa ctgtggcgcg aagccctcaa gcaacacagc gatccggtac tggcgtgggc atcgattatg 
0421 aacgatccgc aaaagtgcct gagctacaaa caagtgcgtg ggcgcggcgg gtttatccgc tccaactggc 
0491 aggaactaaa ccagctgatt gccgccgcta acgtctggac catcaaaacc tacggcccgg atcgcgttgc 
0561 cggtttctcg ccgatcccgg cgatgtcgat ggtttcttac gccgccggaa cgcgttatct gtcgctgctt 
0631 ggcggcacct gtttaagttt ctacgactgg tattgcgacc tgccgcccgc ctcgccgatg acctggggcg 
0701 agcaaaccga cgtaccggaa tctgccgact ggtataactc cagctacatc atcgcctggg ggtctaacgt 
0771 accgcagaca cgtacgccgg acgcccactt ctttaccgaa gtacgctaca aaggcactaa aaccatcgcc 
0841 attacccctg actactctga agtggccaaa ttgtgcgacc agtggctggc accgaaacaa ggcactgata 
0911 gcgccctggc gatggcaatg ggccatgtga ttttaaaaga gtttcatctc gataatccca gcgactactt 
0981 tatcaactac tgccgccgct acagcgacat gccgatgctg gtaatgctgg agcctcgcga cgatggtagc 
1051 tacgttcccg ggcgcatgat ccgcgcatct gacctggtgg atggactggg cgaaagcaac aatccgcagt 
1121 ggaaaaccgt agcagttaat accgcaggtg aattggtagt gccgaacggt tcgattggtt tccgctgggg 
1191 agaaaaaggc aaatggaatc tggaatccat tgccgccggt acggaaaccg aattgtcgtt aaccctgctc 
1261 ggtcaacatg acgctgttgc aggcgtggcc ttcccctact ttggcggcat tgaaaatccg cattttcgca 
1331 gcgtaaaaca caatccggtg ctggtgcgcc aattgcccgt taaaaacctg acgttagtcg atggcaacac 
1401 ctgtccggtg gtcagcgttt atgatttggt actggcgaat tacggcctcg atcgcgggct ggaagatgaa 
1471 aacagtgcga aagattacgc tgaaatcaaa ccgtacaccc cagcctgggg tgagcaaatt accggcgtgc 
1541 cgcgccagta tattgaaacc atcgcccgtg aatttgccga taccgcccat aaaacgcatg ggcgctcgat 
1611 gattatcctc ggcgcaggtg ttaaccactg gtatcacatg gacatgaact accgcgggat gatcaatatg 
1681 ctgatcttct gcggttgtgt cgggcaaagc ggtggcggct gggcacacta tgtcggccag gaaaaactgc 
1751 gcccacaaac cggctggttg ccactggcct ttgcgctcga ctggaaccga ccaccgcgcc aaatgaacag 
1821 cacctcgttt ttctacaatc attccagcca atggcgctat gaaaaagtct ctgctcagga gttactttca 
1891 ccgctcgccg atgccagtaa gtacagcggt catctgattg atttcaacgt tcgcgccgaa cgtatgggct 
1961 ggctaccttc tgcgccgcag ctggggcgta acccgctcgg gattaaagct gaagccgaca aggccggatt 
2031 atcccccaca gaatttaccg cccaggcgct gaaatcgggc gatttacgta tggcctgcga acaaccagat 
2101 agcagcagca atcatccgcg taatttgttt gtctggcgct ctaacctgct tggctcctcc ggcaaaggcc 
2171 acgagtatat gcagaagtat ctgctgggga ccgaaagcgg gattcagggc gaggaactcg gtgccagcga 
2241 cgggatcaaa ccggaagaag tcgagtggca aactgcagcg attgaaggca agctcgacct gctggtgacg 
2311 ctcgacttcc gcatgtccag tacctgcctg ttctccgata tcgttctgcc caccgccacc tggtacgaaa 
2381 aagacgatat gaacacctcg gatatgcatc cgtttattca tccgctttct gcggcggtcg atccggcgtg 
2451 ggaatcacgc agcgactggg aaatctacaa aggtatcgcc aaagcatttt cgcaagtgtg cgtgggtcat 
2521 cttggcaaag aaaccgacgt ggtattacaa cccctactgc atgactctcc ggcagagctc tcacagccgt 
2591 gtgaagtgct cgactggcgc aaaggcgaat gcgatctgat tccaggcaaa accgcgccga atattgtggc 
2661 ggtggagcgc gactaccctg ctacgtatga acgctttacc tcgctcgggc cattgatgga caaacttggc 
2731 aatggcggta aagggatttc gtggaatacc caggatgaaa tcgatttcct cggcaaactc aattacacca 
2801 agcgtgatgg cccggcacag gggcgtccgc tgattgacac cgccattgac gcttcagaag tgattctggc 
2871 actggcaccg gaaaccaacg gtcatgttgc agtcaaagcg tggcaggcgc tgggcgagat caccgggcgc 
2941 gaacataccc atctggcgct gcacaaagag gacgagaaga ttcgctttcg cgatattcag gcgcagccgc 
3011 gtaaaattat ctccagcccc acctggtccg gtctggaaag cgatcacgtc tcctataacg cgggatacac 
3081 caacgttcat gagttaattc cgtggcgcac gctgtcggga cgccagcagc tctatcagga tcatccgtgg 
3151 atgcgtgctt ttggtgaaag cctggtggct tatcgcccgc ctatcgacac ccgtagcgtc agtgagatgc 
3221 gccagatacc gccaaacggc ttcccggaaa aagcacttaa cttcctgacg ccgcaccaga aatggggcat 
3291 tcactcaacc tacagtgaaa acctgctaat gctgacgctc tctcgcggtg gaccgattgt ctggatcagc 
3361 gaaacagatg cccgagaact gaccattgtc gataacgact gggtggaagt attcaacgcc aacggcgcgc 
3431 tgactgcccg cgcggtggtc agccaacgtg taccgccggg catgaccatg atgtatcacg cccaggaacg 
3501 cattatgaat attcctggtt cggaagtaac tggcatgcgc ggcggcattc ataactcggt tacccgcgtt 
3571 tgcccgaaac caacgcatat gattggcggt tacgcgcagc tggcctgggg ctttaactac tacggcaccg 
3641 tcggatcgaa ccgcgatgag ttcatcatga tccgcaagat gaagaacgtt aactggctgg atgatgaagg 
3711 tcgcgatcag gtacaggagg cgaaaaaatg aaaatccgtt cacaagtcgg catggtgctt aacctcgaca 
3781 aatgtatcgg ctgccatacc tgttcggtga cctgtaaaaa cgtctggacc gggcgcgaag g