MG1655
W3110
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Gene Report : fdnI
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Mol Microbiol. 1996;20(3):621-32.
Analysis of nitrate regulatory protein NarL-binding sites in the fdnG and narG operon control regions of Escherichia coli K-12.
Darwin AJ, Li J, Stewart V. ( 8736541 )
     Deletion 20 (LD)  ,  DE(PLK1608)  ,  OCR70,L90-5 (LD 20-1_P/O) (D)  ,  OCR70,L90-6 (LD 20-1_P/O & 20-2TC) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  fdnI
Related strains
     Strains Search Search strains by gene name:  fdnI   Search strains by all related name:  fdnI b1476 ECK1470 JW1472 o217

General information  (Go to Linear View:)
 Gene Name fdnI  
 Alternative name b1476,ECK1470,JW1472,o217  
 Location, Length 1,549,362 - 1,550,015 (  +  ) ;   33.39 min ; 654 (bp) ,   217 (aa) Go to Linear View
 Product formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible  
 Operon Name fdnGHI  
 Note formate dehydrogenase-N, nitrate-inducible, cytochrome B556(Fdn) gamma subunit; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009061 - anaerobic respiration; GO_process: GO:0017004 - cytochrome complex assembly  
 Function enzyme; Energy metabolism, carbon: Anaerobic respiration  
 Gene Ontology GO:0005506 ; iron ion binding ( fdnI )
GO:0005886 ; plasma membrane ( fdnI )
GO:0006118 ; electron transport ( fdnI )
GO:0006810 ; transport ( fdnI )
GO:0008863 ; formate dehydrogenase activity ( fdnI )
GO:0009326 ; formate dehydrogenase complex ( fdnI )
GO:0016020 ; membrane ( fdnI )
GO:0016021 ; integral to membrane ( fdnI )
GO:0045333 ; cellular respiration ( fdnI )
GO:0046872 ; metal ion binding ( fdnI )
 PID 1787750  
 EC number
  (KEGG Pathway)
 
 
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) FDNI_ECOLI ( P24185 ; P77513 )
    -  Protein name Formate dehydrogenase, nitrate-inducible, cytochrome b556(FDN) subunit  
    -  Synonyms Formate dehydrogenase-N gamma subunit, FDH-N gamma subunit, Anaerobic formate dehydrogenase cytochrome b556 subunit  
    -  Gene name Name=fdnI; OrderedLocusNames=b1476, z2234, ECs2080, SF1749, S1882;  

 Linear View (Whole Mode)
View Location
   1540.0  –  1565.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP fdnI (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain C, Formate Dehydrogenase N From E. Coli  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Formate dehydrogenase, nitrate-inducible, cytochrome b556(fdn) subunit (Formate dehydrogenase-N subunit gamma) (FDH-N subunit gamma) (Anaerobic formate dehydrogenase cytochrome b556 subunit)  
         PSI-BLAST Formate dehydrogenase, cytochrome b556 subunit
    nr     (database updated : 2007.02.20 )
         BLAST formate dehydrogenase-N, nitrate-inducible, cytochrome B556(Fdn) gamma subunit [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG2864C 
    EcoCyc fdnI 

MMBR References
J Bacteriol. 1992;174:4935-4942
Localization of upstream sequence elements required for nitrate and anaerobic induction of fdn (formate dehydrogenase-N) operon expression in Escherichia coli K-12.
Li, J., and V. J. Stewart.
J Biol Chem. 1991;266:22386-22391
Nitrate-inducible formate dehydrogenase in Escherichia coli K-12. II. Evidence that a mRNA stem-loop structure is essential for decoding opal (UGA) as selenocysteine.
Berg, B. L., C. Baron, and V. J. Stewart.
J Biol Chem. 1991;266:22380-22385
Nitrate-inducible formate dehydrogenase in Escherichia coli K-12. I. Nucleotide sequence of the fdnGHI operon and evidence that opal (UGA) encodes selenocysteine.
Berg, B. L., J. Li, J. Heider, and V. J. Stewart.
Genetics 1990;125:691-702
Structural genes for nitrate-inducible formate dehydrogenase in Escherichia coli K-12.
Berg, B. L., and V. J. Stewart.
J Bacteriol. 1993;175:3259-3268
Dual response regulators (NarL and NarP) interact with dual sensors (NarX and NarQ) to control nitrate- and nitrite-regulated gene expression in Escherichia coli K-12.
Rabin, R. S., and V. J. Stewart.
Genes Dev. 1994;8:376-385
Recognition of the mRNA selenocysteine insertion sequence by the specialized translational elongation factor SELB.
Ringquist, S., D. Schneider, T. Gibson, C. Baron, A. Bock, and L. Gold.
J Bacteriol. 1991;173(14):4417-23.
Genetic evidence that genes fdhD and fdhE do not control synthesis of formate dehydrogenase-N in Escherichia coli K-12.
Stewart V, Lin JT, Berg BL. ( 1648557 )

Sequences
Amino acid
FASTA format
0001 MSKSKMIVRT KFIDRACHWT VVICFFLVAL SGISFFFPTL QWLTQTFGTP QMGRILHPFF GIAIFVALMF 
0071 MFVRFVHHNI PDKKDIPWLL NIVEVLKGNE HKVADVGKYN AGQKMMFWSI MSMIFVLLVT GVIIWRPYFA 
0141 QYFPMQVVRY SLLIHAAAGI ILIHAILIHM YMAFWVKGSI KGMIEGKVSR RWAKKHHPRW YREIEKAEAK 
0211 KESEEGI

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             CTGGCAGCGG CTGGCTTTAT TGCCACTTTT 
-070 GCCGGGTTGA TTTTCCACTA CATCGGTATT GGCCCGAATA AGGAAGTGGA CGATGACGAG GAGGATCATC 
0001 atgagtaagt cgaaaatgat tgtgcgcacc aaatttattg atcgcgcctg tcactggacc gtggtgattt 
0071 gcttcttcct ggtggcgctg tccgggattt cgttcttctt cccgacgctg caatggctga cgcaaacctt 
0141 cggtacgccg cagatgggac gcattttgca cccgttcttc ggcattgcga ttttcgtcgc actgatgttt 
0211 atgtttgtgc gttttgtgca tcacaacatc ccggataaga aagatattcc gtggctgttg aacattgtcg 
0281 aagtattgaa aggcaatgag cataaagtgg cggatgtcgg taagtacaac gccgggcaaa agatgatgtt 
0351 ctggtcgatc atgagcatga ttttcgtgct gctggtgacc ggggtgatta tctggcgtcc gtactttgcg 
0421 cagtacttcc cgatgcaggt tgttcgctac agcctgctga tccacgcggc tgcgggtatc atcctgatcc 
0491 acgccatcct gatccatatg tatatggcat tttgggtgaa aggatcgatt aaagggatga tcgaagggaa 
0561 ggtaagtcgt cgctgggcga agaaacacca tccgcgctgg tatcgtgaaa tcgagaaggc agaagcgaaa 
0631 aaagagagtg aagaagggat ataaTCTCTT TTGAACTTTA AGCTGAAAAT GGCGCTGTAA AAGGCGCCAT 
0701 TTTCATATTG TAGACAACGT AGGCTTTGTT CATGCCGGAT GCGGCGTGAA CGCC