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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
|
LD3-5-1
(LD)
,
OCL77
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
rcsA
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Related strains
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Strains Search
|
Search strains by gene name:
rcsA
Search strains by all related name:
rcsA b1951 cpsR ECK1949 JW1935
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General information (Go to Linear View: )
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Gene Name
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rcsA
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Alternative name
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b1951,cpsR,ECK1949,JW1935
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Location, Length
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2,021,992 - 2,022,615
(
+
)
;
43.58
min
;
624
(bp) ,
207
(aa)
|
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Product
|
DNA-binding transcriptional activator, co-regulator with RcsB
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Operon Name
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rcsA
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Note
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positive regulator for ctr capsule biosynthesis, positive transcription factor; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0016563 - transcription activator activity; GO_function: GO:0042280 - cell surface antigen activity, host-interacting; GO_process: GO:0009242 - colanic acid biosynthetic process; GO_process: GO:0009248 - K antigen biosynthetic process; GO_process: GO:0006350 - transcription
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Function
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regulator; Surface polysaccharides & antigens
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Gene Ontology
|
GO:0003677
;
DNA binding ( rcsA )
GO:0003700
;
transcription factor activity ( rcsA )
GO:0005622
;
intracellular ( rcsA )
GO:0006350
;
transcription ( rcsA )
GO:0006355
;
regulation of transcription, DNA-dependent ( rcsA )
GO:0043565
;
sequence-specific DNA binding ( rcsA )
GO:0050896
;
response to stimulus ( rcsA )
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PID
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1788262
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EC number
(KEGG Pathway)
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
|
rcsA
(
EG10820
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
|
RCSA_ECOLI
(
P24210
)
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- Protein name
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Colanic acid capsular biosynthesis activation protein A.
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- Synonyms
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- Gene name
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Name=rcsA; OrderedLocusNames=b1951, z3041, ECs2690;
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MMBR References
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J Bacteriol. 1996;178:5053-5056
Identification of a second RcsA protein, a positive regulator of colanic acid capsular polysaccharide genes, in Escherichia coli.
Dierksen, K. P., and J. E. Trempy.
|
J Bacteriol. 1985;162(3):1111-9.
Regulation of capsular polysaccharide synthesis in Escherichia coli K-12: characterization of three regulatory genes.
Gottesman S, Trisler P, Torres-Cabassa AS.
(
3888955
)
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J Bacteriol. 1996;178:4273-4280
Identification of the promoter region for the colanic acid polysaccharide biosynthetic genes in Escherichia coli K-12.
Stout, V.
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J Bacteriol. 1991;173(5):1738-47.
RcsA, an unstable positive regulator of capsular polysaccharide synthesis.
Stout V, Torres-Cabassa A, Maurizi MR, Gutnick D, Gottesman S.
(
1999391
)
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J Bacteriol. 1987;169(3):981-9.
Capsule synthesis in Escherichia coli K-12 is regulated by proteolysis.
Torres-Cabassa AS, Gottesman S.
(
3029041
)
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| Sequences |
Amino acid
FASTA format
|
0001 MSTIIMDLCS YTRLGLTGYL LSRGVKKREI NDIETVDDLA IACDSQRPSV VFINEDCFIH DASNSQRIKL
0071 IINQHPNTLF IVFMAIANVH FDEYLLVRKN LLISSKSIKP ESLDDILGDI LKKETTITSF LNMPTLSLSR
0141 TESSMLRMWM AGQGTIQISD QMNIKAKTVS SHKGNIKRKI KTHNKQVIYH VVRLTDNVTN GIFVNMR
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CGCGAGAGTG GCGCATTAGG TGACCCATGT
-070 TGTTCCGTTT AGTCATGATG AAATATTCAG GTAAGGGGAA TTATCGTTAC GCATTGAGTG AGGGTATGCC
0001 atgtcaacga ttattatgga tttatgtagt tacacccgac taggtttaac cgggtatctg ttgagtagag
0071 gggttaaaaa aagagaaatc aacgacattg aaaccgttga tgaccttgcc atagcttgtg attcacagcg
0141 cccttcagtg gtgtttatta atgaggactg tttcatccac gatgcttcta acagtcagcg tatcaagctc
0211 atcattaatc aacatcccaa tacgttattt atcgttttta tggcaattgc caatgttcat tttgatgaat
0281 atctattggt cagaaaaaat ttattgatca gttctaaatc gattaaaccg gaatctctcg acgatatcct
0351 tggcgatatt ctgaaaaaag agacaacgat aacctcgttt ttaaatatgc cgacgttatc attgagccga
0421 accgaatcga gtatgttgcg aatgtggatg gcaggtcagg gaaccattca aatctctgac caaatgaata
0491 tcaaagccaa gaccgtttca tcgcataaag gtaatattaa acgtaagatc aaaacgcata ataaacaggt
0561 tatctaccat gtcgtccgac tgacggataa tgtgactaat ggtatttttg tcaacatgcg ctaaCACATT
0631 CTGACTGGTG GTTTCCCACC AGTCAGGCTG AATAAGATTA CTCTGCTTTC TCCACAAAGA TACCGTCCTG
0701 ATGCCCTGCT TCATTAAAGA ACCA
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