|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
J Bacteriol. 1998;180(11):2915-23.
Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12.
Diaz E, Ferrandez A, Garcia JL.
(
9603882
)
|
|
|
Deletion
|
OCL55
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
hcaF
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
hcaF
Search strains by all related name:
hcaF b2539 digB ECK2536 hcaA hcaA2 hcaB JW2523 o172 o172d phdC2 yfhV
|
General information (Go to Linear View: )
|
|
Gene Name
|
hcaF
|
|
Alternative name
|
b2539,digB,ECK2536,hcaA,hcaA2,hcaB,JW2523,o172,o172d,phdC2,yfhV
|
|
Location, Length
|
2,668,412 - 2,668,930
(
+
)
;
57.51
min
;
519
(bp) ,
172
(aa)
|
|
Product
|
3-phenylpropionate dioxygenase, small (beta) subunit
|
|
Operon Name
|
hcaEFCBD
|
|
Note
|
small terminal subunit of phenylpropionate dioxygenase; GO_process: GO:0016052 - carbohydrate catabolic process
|
|
Function
|
enzyme; Degradation of small molecules: Carbon compounds
|
|
Gene Ontology
|
GO:0003824
;
catalytic activity ( hcaB )
GO:0003824
;
catalytic activity ( hcaF )
GO:0005488
;
binding ( hcaB )
GO:0006118
;
electron transport ( hcaF )
GO:0006725
;
aromatic compound metabolic process ( hcaF )
GO:0008152
;
metabolic process ( hcaB )
GO:0008695
;
3-phenylpropionate dioxygenase activity ( hcaF )
GO:0016491
;
oxidoreductase activity ( hcaB )
GO:0016491
;
oxidoreductase activity ( hcaF )
GO:0016702
;
oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen ( hcaF )
GO:0019439
;
aromatic compound catabolic process ( hcaB )
GO:0019439
;
aromatic compound catabolic process ( hcaF )
|
|
PID
|
1788889
|
EC number
(KEGG Pathway)
|
1.14.12.19
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
HCAE_ECOLI
(
Q47139
;
P77590; P78203
)
|
|
- Protein name
|
3-phenylpropionate dioxygenase alpha subunit
|
|
- Synonyms
|
EC 1.14.1.-, Digoxigenin alpha subunit
|
|
- Gene name
|
OrderedLocusNames=b2538, z3809, ECs3404;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
hcaF
(
homologous genes of other bacterias
)
hcaB
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain B, Cumene Dioxygenase
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
3-phenylpropionate dioxygenase subunit beta
|
|
PSI-BLAST
|
Biphenyl dioxygenase subunit beta
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
3-phenylpropionate dioxygenase, small (beta) subunit [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
|
|
MMBR References
|
Ph.D. thesis. University of Minnesota, Minneapolis-St. Paul. 1983
The degradation of 3-phenylpropionic acid by Escherichia coli.
Burlingame, R. P.
|
J Bacteriol. 1986;168(1):55-64.
Isolation and characterization of Escherichia coli mutants defective for phenylpropionate degradation.
Burlingame RP, Wyman L, Chapman PJ.
(
3531186
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSAQVSLELH HRISQFLFHE ASLLDDWKFR DWLAQLDEEI RYTMRTTVNA QTRDRRKGVQ PPTTWIFNDT
0071 KDQLERRIAR LETGMAWAEE PPSRTRHLIS NCQISETDIP NVFAVRVNYL LYRAQKERDE TFYVGTRFDK
0141 VRRLEDDNWR LLERDIVLDQ AVITSHNLSV LF
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CCGCTCGTGG AATGTACCAA CGCTGGGCCG
-070 ATCTTCTGAG TAGCGAAAGC TGGCAGGAAG TGCTCGATAA AACCGCCGCT TACCAGCAGG AGGTGATGAA
0001 atgagtgcgc aagtttcact agagttacat caccgcatta gccagtttct ctttcacgaa gccagcttac
0071 tggacgactg gaaatttcgt gactggctgg cgcagctcga cgaagagatt cgttacacca tgcgcaccac
0141 agttaacgcg caaacacgcg accgccgcaa aggcgtccag ccaccgacaa cctggatttt taatgacacc
0211 aaagaccagc tggagcggcg aatcgcccgt ctggaaacgg gcatggcctg ggcagaagag ccgccgtcac
0281 gcacccgtca cttaatcagc aactgccaga taagcgaaac cgacatccca aacgtatttg ctgtgcgggt
0351 aaattatctg ctttatcggg cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg tttcgacaaa
0421 gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt cctggatcaa gcggtaatca
0491 cttcccataa cctgagtgta ctgttctgaT GAATCGAATT TATGCGTGTC CCGTTGCGGA TGTGCCGGAG
0561 GGTGAGGCTC TCCGGATCGA TACCTCGCCC GTCATCGCCC TGTTCAACGT TGGCGGCGA
|
