|
PEC Original Annotations
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|
Essentiality
|
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Class
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non-essential
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References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
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Deletion
|
OCL53
(D)
,
OCL53-8
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
nadB
|
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Related strains
|
|
Strains Search
|
Search strains by gene name:
nadB
Search strains by all related name:
nadB b2574 ECK2572 JW2558 nic nicB
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General information (Go to Linear View: )
|
|
Gene Name
|
nadB
|
|
Alternative name
|
b2574,ECK2572,JW2558,nic,nicB
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|
Location, Length
|
2,708,442 - 2,710,064
(
+
)
;
58.38
min
;
1623
(bp) ,
540
(aa)
|
|
Product
|
quinolinate synthase, L-aspartate oxidase (B protein) subunit
|
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Operon Name
|
nadB
|
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Note
|
quinolinate synthetase, B protein; GO_process: GO:0009435 - NAD biosynthetic process
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Function
|
enzyme; Biosynthesis of cofactors, carriers: Pyridine nucleotide
|
|
Gene Ontology
|
GO:0005737
;
cytoplasm ( nadB )
GO:0006118
;
electron transport ( nadB )
GO:0008734
;
L-aspartate oxidase activity ( nadB )
GO:0009435
;
NAD biosynthetic process ( nadB )
GO:0016491
;
oxidoreductase activity ( nadB )
GO:0019363
;
pyridine nucleotide biosynthetic process ( nadB )
|
|
PID
|
1788928
|
EC number
(KEGG Pathway)
|
1.4.3.16
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
nadB
(
EG10631
)
|
|
Number of removed
|
0 aa cleaved
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
NADB_ECOLI
(
P10902
;
P78099
)
|
|
- Protein name
|
L-aspartate oxidase
|
|
- Synonyms
|
EC 1.4.3.16, LASPO, Quinolinate synthetase B
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|
- Gene name
|
Name=nadB; Synonyms=nicB; OrderedLocusNames=b2574;
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Homology Analysis
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BLAST
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Bacteria
|
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GTOP
|
nadB
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Structure Of L-Aspartate Oxidase: Implications For The Succinate Dehydrogenase FUMARATE REDUCATSE FAMILY
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
L-aspartate oxidase
|
|
PSI-BLAST
|
L-aspartate oxidase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
L-aspartate oxidase [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
|
|
|
PROSITE
|
|
PROSITE
|
GLYCOSAMINOGLYCAN
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
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MMBR References
|
Eur J Biochem. 1988;175:221-228
Molecular biology of pyridine nucleotide biosynthesis in Escherichia coli. Cloning and characterization of quinolinate synthesis genes nadA and nadB.
Flachmann, R., N. Kunz, J. Seifert, M. Gutlich, F. J. Wientjes, A. Laufer, and H. G. Gassen.
|
Eur J Biochem. 1996;239:418-426
L-Aspartate oxidase from Escherichia coli. I. Characterization of coenzyme binding and product inhibition.
Mortarino, M., A. Negri, G. Tedeschi, T. Simonic, S. Duga, H. G. Gassen, and S. Ronchi.
|
Biol Chem Hoppe Seyler. 1990;371(3):239-48.
Expression of the E. coli nadB gene and characterization of the gene product L-aspartate oxidase.
Seifert J, Kunz N, Flachmann R, Laufer A, Jany KD, Gassen HG.
(
2187483
)
|
Eur J Biochem. 1996;239:427
L-Aspartate oxidase from Escherichia coli. II. Interaction with C4 dicarboxylic acids and identification of a novel L-aspartate: fumarate oxidoreductase activity.
Tedeschi, G., A. Negri, M. Mortarino, F. Ceciliani, T. Simonic, L. Faotto, and S. Ronchi.
|
| Sequences |
Amino acid
FASTA format
|
0001 MNTLPEHSCD VLIIGSGAAG LSLALRLADQ HQVIVLSKGP VTEGSTFYAQ GGIAAVFDET DSIDSHVEDT
0071 LIAGAGICDR HAVEFVASNA RSCVQWLIDQ GVLFDTHIQP NGEESYHLTR EGGHSHRRIL HAADATGREV
0141 ETTLVSKALN HPNIRVLERS NAVDLIVSDK IGLPGTRRVV GAWVWNRNKE TVETCHAKAV VLATGGASKV
0211 YQYTTNPDIS SGDGIAMAWR AGCRVANLEF NQFHPTALYH PQARNFLLTE ALRGEGAYLK RPDGTRFMPD
0281 FDERGELAPR DIVARAIDHE MKRLGADCMF LDISHKPADF IRQHFPMIYE KLLGLGIDLT QEPVPIVPAA
0351 HYTCGGVMVD DHGRTDVEGL YAIGEVSYTG LHGANRMASN SLLECLVYGW SAAEDITRRM PYAHDISTLP
0421 PWDESRVENP DERVVIQHNW HELRLFMWDY VGIVRTTKRL ERALRRITML QQEIDEYYAH FRVSNNLLEL
0491 RNLVQVAELI VRCAMMRKES RGLHFTLDYP ELLTHSGPSI LSPGNHYINR
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CGTGTTTAGC AACTGTAACA AATATTAAAA
-070 TAGCAGGTGT TTATCCGCAC AACATGATGC TATGCTGACC AAACCATGTT TAGTAAATTA AACAAAGAAA
0001 atgaatactc tccctgaaca ttcatgtgac gtgttgatta tcggtagcgg cgcagccgga ctttcactgg
0071 cgctacgcct ggctgaccag catcaggtca tcgttctaag taaaggcccg gtaacggaag gttcaacatt
0141 ttatgcccag ggcggtattg ccgccgtgtt tgatgaaact gacagcattg actcgcatgt ggaagacaca
0211 ttgattgccg gggctggtat ttgcgatcgc catgcagttg aatttgtcgc cagcaatgca cgatcctgtg
0281 tgcaatggct aatcgaccag ggggtgttgt ttgataccca cattcaaccg aatggcgaag aaagttacca
0351 tctgacccgt gaaggtggac atagtcaccg tcgtattctt catgccgccg acgccaccgg tagagaagta
0421 gaaaccacgc tggtgagcaa ggcgctgaac catccgaata ttcgcgtgct ggagcgcagc aacgcggttg
0491 atctgattgt ttctgacaaa attggcctgc cgggcacgcg acgggttgtt ggcgcgtggg tatggaaccg
0561 taataaagaa acggtggaaa cctgccacgc aaaagcggtg gtgctggcaa ccggcggtgc gtcgaaggtt
0631 tatcagtaca ccaccaatcc ggatatttct tctggcgatg gcattgctat ggcgtggcgc gcaggctgcc
0701 gggttgccaa tctcgaattt aatcagttcc accctaccgc gctatatcac ccacaggcac gcaatttcct
0771 gttaacagaa gcactgcgcg gcgaaggcgc ttatctcaag cgcccggatg gtacgcgttt tatgcccgat
0841 tttgatgagc gcggcgaact ggccccgcgc gatattgtcg cccgcgccat tgaccatgaa atgaaacgcc
0911 tcggcgcaga ttgtatgttc cttgatatca gccataagcc cgccgatttt attcgccagc atttcccgat
0981 gatttatgaa aagctgctcg ggctggggat tgatctcaca caagaaccgg taccgattgt gcctgctgca
1051 cattatacct gcggtggtgt aatggttgat gatcatgggc gtacggacgt cgagggcttg tatgccattg
1121 gcgaggtgag ttataccggc ttacacggcg ctaaccgcat ggcctcgaat tcattgctgg agtgtctggt
1191 ctatggctgg tcggcggcgg aagatatcac cagacgtatg ccttatgccc acgacatcag tacgttaccg
1261 ccgtgggatg aaagccgcgt tgagaaccct gacgaacggg tagtaattca gcataactgg cacgagctac
1331 gtctgtttat gtgggattac gttggcattg tgcgcacaac gaagcgcctg gaacgcgccc tgcggcggat
1401 aaccatgctc caacaagaaa tagacgaata ttacgcccat ttccgcgtct caaataattt gctggagctg
1471 cgtaatctgg tacaggttgc cgagttgatt gttcgctgtg caatgatgcg taaagagagt cgggggttgc
1541 atttcacgct ggattatccg gaactgctca cccattccgg tccgtcgatc ctttcccccg gcaatcatta
1611 cataaacaga taaAAAGCCT GGGTCAGCGC CGTATACGCT TCGGAATAGT TCTGGTCTGG CCCACGAATG
1681 ACTAAGCGAT CGCTAAAGCA TTCTCCCGCC TGCGGGGAGA ATG
|
