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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Proc Natl Acad Sci U S A. 1994;91(10):4539-43.
A cytoplasmic domain is important for the formation of a SecY-SecE translocator complex.
Baba T, Taura T, Shimoike T, Akiyama Y, Yoshihisa T, Ito K.
(
8183945
)
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Deletion
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OCL22,23-4 (BK5-12 / AK4-12)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
secY
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Related strains
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Strains Search
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Search strains by gene name:
secY
Search strains by all related name:
secY b3300 ECK3287 f443 JW3262 prlA
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General information (Go to Linear View: )
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Gene Name
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secY
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Alternative name
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b3300,ECK3287,f443,JW3262,prlA
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Location, Length
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3,440,788 - 3,442,119
(
-
)
;
74.16
min
;
1332
(bp) ,
443
(aa)
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Product
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preprotein translocase membrane subunit
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Operon Name
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rplNXE-rpsNH-rplFR-rpsE-rpmD-rplO-secY-rpmJ
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Note
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putative ATPase subunit of translocase; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane
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Function
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putative transport; Not classified
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Gene Ontology
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GO:0005515
;
protein binding ( secY )
GO:0005886
;
plasma membrane ( secY )
GO:0006810
;
transport ( secY )
GO:0009306
;
protein secretion ( secY )
GO:0015031
;
protein transport ( secY )
GO:0015450
;
P-P-bond-hydrolysis-driven protein transmembrane transporter activity ( secY )
GO:0016020
;
membrane ( secY )
GO:0016021
;
integral to membrane ( secY )
GO:0065002
;
intracellular protein transport across a membrane ( secY )
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PID
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1789696
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EC number
(KEGG Pathway)
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SWISS-PROT
(
Show details
)
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Entry name(Acc.no)
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SECY_ECOLI
(
P03844
)
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- Protein name
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Preprotein translocase secY subunit.
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- Synonyms
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- Gene name
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OrderedLocusNames=b3300, c4061, z4670, ECs4165, SF3332, S4430;
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MMBR References
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J Biol Chem. 1995;270:20106-20111
SecYEG and SecA are the stoichiometric components of preprotein translocase.
Douville, K., A. Price, J. Eichler, A. Economou, and W. Wickner.
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Cell. 1981;23(1):79-88.
Suppressor mutations that restore export of a protein with a defective signal sequence.
Emr SD, Hanley-Way S, Silhavy TJ.
(
7011570
)
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J Bacteriol. 1994;176:5607-5614
PrlA and PrlG suppressors reduce the requirement for signal sequence recognition.
Flower, A. M., R. C. Doebele, and T. J. Silhavy.
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J Bacteriol. 1993;175:4036-4044
prlA supression of defective export of maltose-binding protein in secB mutants of Escherichia coli.
Francetic, O., M. P. Hanson, and C. A. Kumamoto.
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J Bacteriol. 1990;172(12):7005-10.
Characterization of cold-sensitive secY mutants of Escherichia coli.
Baba T, Jacq A, Brickman E, Beckwith J, Taura T, Ueguchi C, Akiyama Y, Ito K.
(
2254269
)
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EMBO J. 1984;3:2319-2324
Characterization of an amber mutation in the structural gene for ribosomal protein L15, which impairs the expression of the protein export gene, secY, in Escherichia coli.
Ito, K., D. P. Cerretti, H. Nashimoto, and M. Nomura.
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Cell. 1983;32(3):789-97.
A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins.
Ito K, Wittekind M, Nomura M, Shiba K, Yura T, Miura A, Nashimoto H.
(
6339072
)
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J Bacteriol. 1989;171(3):1742-3.
Temperature-sensitive sec mutants of Escherichia coli: inhibition of protein export at the permissive temperature.
Ito K, Hirota Y, Akiyama Y.
(
2646297
)
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J Bacteriol. 1993;175:6113-6117
Processing of lipid-modified prolipoprotein requires energy and sec gene products in vivo.
Kosic, N., M. Sugai, C. K. Fan, and H. C. Wu.
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Proc Natl Acad Sci U S A. 1996;93:5953-5957
prlA suppressors in Escherichia coli relieve the proton electrochemical gradient dependency of translocation of wild-type precursors.
Nouwen, N., B. de Kruijff, and J. Tommassen.
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J Bacteriol. 1993;175:7092-7096
Isolation and analysis of novel mutants of Escherichia coli prlA (secY).
Olsen, M. K., E. L. Rosey, and C.-S. C. Tomich.
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EMBO J. 1993;12:3391-3398
PrlA suppressor mutations cluster in regions corresponding to three distinct topological domains.
Osborne, R. S., and T. J. Silhavy.
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J Biol Chem. 1996;271:19908-19914
In vivo analyses of interactions between SecE and SecY, core components of the Escherichia coli protein translocation machinery.
Pohlschroder, M., C. Murphy, and J. R. Beckwith.
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J Bioenerg Biomembr. 1990;22(3):291-310.
The sec and prl genes of Escherichia coli.
Bieker KL, Phillips GJ, Silhavy TJ.
(
2202721
)
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Cell. 1990;61(5):833-42.
PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coli.
Bieker KL, Silhavy TJ.
(
2111734
)
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Mol Microbiol. 1991;5(2):343-52.
The general protein-export pathway is directly required for extracellular pullulanase secretion in Escherichia coli K12.
Pugsley AP, Kornacker MG, Poquet I.
(
2041472
)
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J Biol Chem. 1996;271:31196-31201
FtsH (HflB) is an ATP-dependent protease selectively acting on SecY and some other membrane proteins.
Akiyama, Y., A. Kihara, H. Tokuda, and K. Ito.
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EMBO J. 1984;3:631-635
A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: isolation and characterization of a new temperature-sensitive secY mutant.
Shiba, K., K. Ito, T. Yura, and D. P. Cerretti.
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Mol Microbiol. 1992;6(9):1205-10.
SecY variants that interfere with Escherichia coli protein export in the presence of normal secY.
Shimoike T, Akiyama Y, Baba T, Taura T, Ito K.
(
1588819
)
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Cell. 1982;31(1):227-35.
A previously unidentified gene in the spc operon of Escherichia coli K12 specifies a component of the protein export machinery.
Shultz J, Silhavy TJ, Berman ML, Fiil N, Emr SD.
(
6297749
)
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EMBO J. 1985;4:3351-3356
The secY membrane component of the bacterial protein export machinery: analysis by new electrophoretic methods for integral membrane proteins.
Akiyama, Y., and K. Ito.
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Biochem Biophys Res Commun. 1990;167(2):711-5.
SecY protein, a membrane-embedded secretion factor of E. coli, is cleaved by the ompT protease in vitro.
Akiyama Y, Ito K.
(
2182019
)
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J Bacteriol. 1990;172(6):2888-93.
SecY, a multispanning integral membrane protein, contains a potential leader peptidase cleavage site.
Akiyama Y, Inada T, Nakamura Y, Ito K.
(
2188944
)
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J Bacteriol. 1997;179:7386-7393
Differential translocation of protein precursors across SecY-deficient membranes of Escherichia coli: SecY is not obligatorily required for translocation of certain secretory proteins in vitro.
Yang, Y. B., J. Lian, and P. C. Tai.
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Nucleic Acids Res. 1983;11:2599-2616
The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene.
Cerretti, D. P., D. Dean, G. R. Davis, D. M. Bedwell, and M. Nomura.
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J Bacteriol. 1995;177:4969-4973
Multicopy suppression of cold-sensitive sec mutations in Escherichia coli.
Danese, P. N., C. K. Murphy, and T. J. Silhavy.
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| Sequences |
Amino acid
FASTA format
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0001 MAKQPGLDFQ SAKGGLGELK RRLLFVIGAL IVFRIGSFIP IPGIDAAVLA KLLEQQRGTI IEMFNMFSGG
0071 ALSRASIFAL GIMPYISASI IIQLLTVVHP TLAEIKKEGE SGRRKISQYT RYGTLVLAIF QSIGIATGLP
0141 NMPGMQGLVI NPGFAFYFTA VVSLVTGTMF LMWLGEQITE RGIGNGISII IFAGIVAGLP PAIAHTIEQA
0211 RQGDLHFLVL LLVAVLVFAV TFFVVFVERG QRRIVVNYAK RQQGRRVYAA QSTHLPLKVN MAGVIPAIFA
0281 SSIILFPATI ASWFGGGTGW NWLTTISLYL QPGQPLYVLL YASAIIFFCF FYTALVFNPR ETADNLKKSG
0351 AFVPGIRPGE QTAKYIDKVM TRLTLVGALY ITFICLIPEF MRDAMKVPFY FGGTSLLIVV VVIMDFMAQV
0421 QTLMMSSQYE SALKKANLKG YGR
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 gaagtaacga ctccggtaac tgttcgtggc
-070 ctgcgtgtta ctaaaggcgc tcgtgctgct atcgaagctg ctggcggtaa aatcgaggaa taagtagcag
0001 atggctaaac aaccgggatt agattttcaa agtgccaaag gtggcttagg cgagctgaaa cgcagactgc
0071 tgtttgttat cggtgcgctg attgtgttcc gtattggctc ttttattccg atccctggta ttgatgccgc
0141 tgtacttgcc aaactgcttg agcaacagcg aggcaccatc attgagatgt ttaacatgtt ctctggtggt
0211 gctctcagcc gtgcttctat ctttgctctg gggatcatgc cgtatatttc ggcgtcgatc attatccagc
0281 tgctgacggt ggttcaccca acgttggcag aaattaagaa agaaggggag tctggtcgtc gtaagatcag
0351 ccagtacacc cgctacggta ctctggtgct ggcaatattc cagtcgatcg gtattgctac cggtctgccg
0421 aatatgcctg gtatgcaagg cctggtgatt aacccgggct ttgcattcta cttcaccgct gttgtaagtc
0491 tggtcacagg aaccatgttc ctgatgtggt tgggcgaaca gattactgaa cgaggtatcg gcaacggtat
0561 ttcaatcatt atcttcgccg gtattgtcgc gggactcccg ccagccattg cccatactat cgagcaagcg
0631 cgtcaaggcg acctgcactt cctcgtgttg ctgttggttg cagtattagt atttgcagtg acgttctttg
0701 ttgtatttgt tgagcgtggt caacgccgca ttgtggtaaa ctacgcgaaa cgtcagcaag gtcgtcgtgt
0771 ctatgctgca cagagcacac atttaccgct gaaagtgaat atggcggggg taatcccggc aatcttcgct
0841 tccagtatta ttctgttccc ggcgaccatc gcgtcatggt tcgggggcgg tactggttgg aactggctga
0911 caacaatttc gctgtatttg cagcctgggc aaccgcttta tgtgttactc tatgcgtctg caatcatctt
0981 cttctgtttc ttctacacgg cgttggtttt caacccgcgt gaaacagcag ataacctgaa gaagtccggt
1051 gcatttgtac caggaattcg tccgggagag caaacggcga agtatatcga taaagtaatg acccgcctga
1121 ccctggttgg tgcgctgtat attaccttta tctgcctgat cccggagttc atgcgtgatg caatgaaagt
1191 accgttctac ttcggtggga cctcactgct tatcgttgtt gtcgtgatta tggactttat ggctcaagtg
1261 caaactctga tgatgtccag tcagtatgag tctgcattga agaaggcgaa cctgaaaggc tacggccgat
1331 aattggtcgc ccgagaagtt acggagagta aaaatgaaag ttcgtgcttc cgtcaagaaa ttatgccgta
1401 actgcaaaat cgttaagcgt gatggtgtca tc
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