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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
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OCL17
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
nikA
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Related strains
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Strains Search
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Search strains by gene name:
nikA
Search strains by all related name:
nikA b3476 ECK3460 hydC hydD JW3441 o524
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General information (Go to Linear View: )
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Gene Name
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nikA
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Alternative name
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b3476,ECK3460,hydC,hydD,JW3441,o524
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Location, Length
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3,611,690 - 3,613,264
(
+
)
;
77.84
min
;
1575
(bp) ,
524
(aa)
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Product
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nickel-binding, heme-binding periplasmic protein
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Operon Name
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nikABCDER
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Note
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periplasmic binding protein for nickel; periplasmic-binding component of ABC superfamily; GO_component: GO:0042597 - periplasmic space; GO_process: GO:0042330 - taxis
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Function
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transport; Transport of small molecules: Cations
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Gene Ontology
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GO:0005215
;
transporter activity ( nikA )
GO:0005515
;
protein binding ( nikA )
GO:0006810
;
transport ( nikA )
GO:0016151
;
nickel ion binding ( nikA )
GO:0042597
;
periplasmic space ( nikA )
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PID
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1789887
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EC number
(KEGG Pathway)
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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NIKA_ECOLI
(
P33590
)
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- Protein name
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Nickel-binding periplasmic protein precursor.
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- Synonyms
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- Gene name
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Name=nikA; OrderedLocusNames=b3476;
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MMBR References
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J Bacteriol. 1995;177:46-51
Specific binding of the NikA protein to one arm of 17-base-pair inverted repeat sequences within the oriT region of plasmid R64.
Furuya, N., and T. Komano.
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Mol Microbiol. 1993;9(6):1181-91.
The nik operon of Escherichia coli encodes a periplasmic binding-protein-dependent transport system for nickel.
Navarro C, Wu LF, Mandrand-Berthelot MA.
(
7934931
)
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J Bacteriol. 1989;171(2):831-6.
Randomly induced Escherichia coli K-12 Tn5 insertion mutants defective in hydrogenase activity.
Stoker K, Oltmann LF, Stouthamer AH.
(
2536683
)
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Gene. 1991;107(1):37-42.
The hydC region contains a multi-cistronic operon (nik) involved in nickel transport in Escherichia coli.
Wu LF, Navarro C, Mandrand-Berthelot MA.
(
1743519
)
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Biochimie. 1986;68(1):167-79.
Genetic and physiological characterization of new Escherichia coli mutants impaired in hydrogenase activity.
Wu LF, Mandrand-Berthelot MA.
(
3089308
)
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Mol Microbiol. 1989;3(12):1709-18.
Nickel deficiency gives rise to the defective hydrogenase phenotype of hydC and fnr mutants in Escherichia coli.
Wu LF, Mandrand-Berthelot MA, Waugh R, Edmonds CJ, Holt SE, Boxer DH.
(
2695744
)
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| Sequences |
Amino acid
FASTA format
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0001 MLSTLRRTLF ALLACASFIV HAAAPDEITT AWPVNVGPLN PHLYTPNQMF AQSMVYEPLV KYQADGSVIP
0071 WLAKSWTHSE DGKTWTFTLR DDVKFSNGEP FDAEAAAENF RAVLDNRQRH AWLELANQIV DVKALSKTEL
0141 QITLKSAYYP FLQELALPRP FRFIAPSQFK NHETMNGIKA PIGTGPWILQ ESKLNQYDVF VRNENYWGEK
0211 PAIKKITFNV IPDPTTRAVA FETGDIDLLY GNEGLLPLDT FARFSQNPAY HTQLSQPIET VMLALNTAKA
0281 PTNELAVREA LNYAVNKKSL IDNALYGTQQ VADTLFAPSV PYANLGLKPS QYDPQKAKAL LEKAGWTLPA
0351 GKDIREKNGQ PLRIELSFIG TDALSKSMAE IIQADMRQIG ADVSLIGEEE SSIYARQRDG RFGMIFHRTW
0421 GAPYDPHAFL SSMRVPSHAD FQAQQGLADK PLIDKEIGEV LATHDETQRQ ALYRDILTRL HDEAVYLPIS
0491 YISMMVVSKP ELGNIPYAPI ATEIPFEQIK PVKP
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CGACTGCCCA TCTATTGATC CAGAACAGGT
-070 AATCAGTATG ACGAATACTT AAAATCGTCA TACTTATTTC CGCCATCTAT TTTAATCCAT TGGGGTTACC
0001 atgctctcca cactccgccg cactctattt gcgctgctgg cttgtgcgtc ttttatcgtc catgccgctg
0071 caccagatga aatcaccacc gcctggccgg tgaatgtcgg gccactaaac ccgcaccttt acacgcctaa
0141 ccagatgttc gcccagagca tggtttatga accattggtg aaatatcagg cagacggttc ggtgatcccg
0211 tggctggcaa aaagctggac tcattcagaa gatggtaaaa cctggacctt caccctgcgt gatgacgtga
0281 aattctccaa cggtgaaccg ttcgatgccg aggcggcggc agaaaacttc cgcgcagtgc tcgataaccg
0351 tcaacgtcac gcctggctgg agctggcaaa ccagattgtt gatgttaaag cactcagtaa aacagagctg
0421 caaattaccc tgaaaagcgc ctactatcct ttcctgcaag aactggccct gccccgtcct ttccgtttta
0491 tcgctccctc gcagtttaaa aaccatgaaa ccatgaacgg aattaaagcg ccgattggca ccggaccgtg
0561 gattttgcag gaatcgaaac tgaatcagta cgatgtcttc gtccgtaacg aaaactactg gggcgaaaag
0631 ccagcgatta aaaagatcac ctttaacgtc atcccggacc cgactacccg cgcggtggcg tttgaaactg
0701 gcgatatcga cctgctgtac ggaaacgaag ggttattacc gctcgatacc ttcgcccgct ttagccagaa
0771 tccggcttac cacacccaac tgtcacagcc gatcgaaacc gtgatgctgg cgctcaatac cgccaaagcc
0841 cccaccaacg agctggcagt acgtgaagct cttaattacg cggtaaacaa aaaatcgctg attgataacg
0911 cgttgtatgg cacccagcag gtcgccgaca ccctgtttgc cccttctgtg ccctacgcca acctcggcct
0981 gaaaccgagc cagtacgatc cgcaaaaagc gaaagcgttg ctggaaaaag ccggttggac gctgcctgcg
1051 ggcaaagaca tccgcgagaa aaatggtcag ccgctgcgca ttgaactttc gttcatcggc accgatgcgt
1121 taagcaaatc gatggcggaa atcattcagg ctgatatgcg ccagattggc gcagatgtct cgctgattgg
1191 cgaagaagag agcagtatct atgctcgtca gcgcgacggt cgttttggca tgattttcca ccgcacctgg
1261 ggcgcgccat atgatccaca cgccttcctc agttcaatgc gcgtaccgtc acacgctgac ttccaggcac
1331 agcaaggatt agccgacaaa ccgctgattg ataaagagat cggcgaagtg ctggcgaccc atgacgaaac
1401 gcaacgtcag gcgctgtatc gcgacattct gacccgtctg catgacgagg cggtttatct gcctatcagt
1471 tacatctcaa tgatggtggt atcaaaaccg gagctgggta acatccccta cgcgccgatc gccaccgaaa
1541 ttccgttcga acagattaaa ccggtgaaac cttaaTGTTG CGTTACGTAT TACGCCGCTT TCTGCTGCTG
1611 ATCCCGATGG TGCTCGCCGC CTCGGTGATC ATTTTTCTGA TGCTGCGCCT CGGTACCGGC GACCC
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