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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Deletion
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OCR03
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
hemD
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Related strains
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Strains Search
|
Search strains by gene name:
hemD
Search strains by all related name:
hemD b3804 ECK3798 JW3776
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General information (Go to Linear View: )
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Gene Name
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hemD
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Alternative name
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b3804,ECK3798,JW3776
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Location, Length
|
3,987,111 - 3,987,851
(
-
)
;
85.94
min
;
741
(bp) ,
246
(aa)
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Product
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uroporphyrinogen III synthase
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Operon Name
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hemCDXY
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Note
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Function
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enzyme; Biosynthesis of cofactors, carriers: Heme, porphyrin
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Gene Ontology
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GO:0004852
;
uroporphyrinogen-III synthase activity ( hemD )
GO:0006779
;
porphyrin biosynthetic process ( hemD )
GO:0016829
;
lyase activity ( hemD )
GO:0033014
;
tetrapyrrole biosynthetic process ( hemD )
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PID
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1790236
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EC number
(KEGG Pathway)
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4.2.1.75
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
|
HEM4_ECOLI
(
P09126
;
Q47250
)
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- Protein name
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Uroporphyrinogen-III synthase
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- Synonyms
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EC 4.2.1.75, UROS, Uroporphyrinogen- III cosynthetase, Hydroxymethylbilane hydrolyase [cyclizing]
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- Gene name
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Name=hemD; OrderedLocusNames=b3804;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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hemD
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, Crystal Structure Of Methanocaldococcus Jannaschii Nucleoside Kinase - An Archaeal Member Of The Ribokinase Family
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Uroporphyrinogen-III synthase
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PSI-BLAST
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Uroporphyrinogen-III synthase
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nr
(database updated :
2007.02.20
)
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BLAST
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unnamed protein product [Escherichia coli]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
AMIDATION
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MMBR References
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Nucleic Acids Res. 1987;15(24):10583
Nucleotide sequence of hemD, the second gene in the hem operon of Escherichia coli K-12.
Jordan PM, Mgbeje BI, Alwan AF, Thomas SD.
(
3320969
)
|
Biochem J. 1988;249(2):613-6.
Nucleotide sequence for the hemD gene of Escherichia coli encoding uroporphyrinogen III synthase and initial evidence for a hem operon.
Jordan PM, Mgbeje BI, Thomas SD, Alwan AF.
(
3277628
)
|
Biochem Biophys Res Commun. 1982;109(2):414-22.
Fine structure of the recB and recC gene region of Escherichia coli.
Sasaki M, Fujiyoshi T, Shimada K, Takagi Y.
(
6295393
)
|
J Bacteriol. 1987;169(9):4257-62.
Molecular cloning and sequencing of the hemD gene of Escherichia coli K-12 and preliminary data on the Uro operon.
Sasarman A, Nepveu A, Echelard Y, Dymetryszyn J, Drolet M, Goyer C.
(
3040684
)
|
J Bioenerg Biomembr. 1995;27(2):181-95.
Porphobilinogen deaminase and uroporphyrinogen III synthase: structure, molecular biology, and mechanism.
Shoolingin-Jordan PM.
(
7592565
)
|
Science. 1992;257(5071):771-8.
Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes.
Daniels DL, Plunkett G 3rd, Burland VD, Blattner FR.
(
1379743
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSILVTRPSP AGEELVSRLR TLGQVAWHFP LIEFSPGQQL PQLADQLAAL GESDLLFALS QHAVAFAQSQ
0071 LHQQDRKWPR LPDYFAIGRT TALALHTVSG QKILYPQDRE ISEVLLQLPE LQNIAGKRAL ILRGNGGREL
0141 IGDTLTARGA EVTFCECYQR CAIHYDGAEE AMRWQAREVT MVVVTSGEML QQLWSLIPQW YREHWLLHCR
0211 LLVVSERLAK LARELGWQDI KVADNADNDA LLRALQ
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 cgcaagatgc cgaacaaatg gggatttcgc
-070 tggcagaaga gctactgaat aacggcgcgc gcgagatcct cgctgaagtc tataacggag acgccccggc
0001 atgagtatcc ttgtcacccg cccgtctccc gctggagaag agttagtgag ccgtctgcgc acactggggc
0071 aggtggcctg gcattttccg ctgattgagt tttctccggg tcaacaatta ccgcaacttg ctgatcaact
0141 ggcagcgctg ggggagagcg atctgttgtt tgccctctcg caacacgcgg ttgcttttgc ccaatcacag
0211 ctgcatcagc aagatcgtaa atggccccga ctacctgatt atttcgccat tggacgcacc accgcactgg
0281 cactacatac cgtaagtgga cagaagattc tctacccgca ggatcgggaa atcagcgaag tcttgctaca
0351 attacctgaa ttacaaaata ttgcgggcaa acgtgcgctg atattacgtg gcaatggtgg tcgtgagcta
0421 attggggata ccctgacggc gcgcggtgct gaggtcactt tttgtgaatg ttatcaacga tgcgcaatcc
0491 attacgatgg tgcagaagaa gcgatgcgct ggcaagcccg cgaggtgacg atggtcgttg ttaccagcgg
0561 tgaaatgttg cagcaactct ggtcgctgat cccacaatgg tatcgtgagc actggttact acactgtcga
0631 ctattggtcg tcagtgagcg tttggcgaaa ctcgcccggg aactgggctg gcaagacatt aaggtcgccg
0701 ataacgctga caacgatgcg cttttacggg cattacaata actctcataa caggaagcca taatgacgga
0771 acaagaaaaa acctccgccg tggttgaaga gaccagggag gccgtggaca ccacgtcaca acctgtcgca
0841 a
|
