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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
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OCR10,11-11 (AK1-16 / BK2-16)
(D)
,
OCR10,11-16 (BK5-16 / AK7-16)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
birA
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Related strains
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Strains Search
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Search strains by gene name:
birA
Search strains by all related name:
birA b3973 bioR dhbB ECK3965 JW3941
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General information (Go to Linear View: )
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Gene Name
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birA
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Alternative name
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b3973,bioR,dhbB,ECK3965,JW3941
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Location, Length
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4,171,105 - 4,172,070
(
+
)
;
89.90
min
;
966
(bp) ,
321
(aa)
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Product
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bifunctional biotin-[acetylCoA carboxylase]holoenzyme synthetase/ DNA-binding transcriptional repressor, bio-5'-AMP-binding
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Operon Name
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murB-birA
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Note
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biotin-[acetylCoA carboxylase] holoenzyme synthetase and biotin operon repressor; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0016564 - transcription repressor activity; GO_process: GO:0009102 - biotin biosynthetic process; GO_process: GO:0006350 - transcription
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Function
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enzyme; Biosynthesis of cofactors, carriers: Biotin
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Gene Ontology
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GO:0003677
;
DNA binding ( birA )
GO:0003824
;
catalytic activity ( birA )
GO:0004077
;
biotin-[acetyl-CoA-carboxylase] ligase activity ( birA )
GO:0005515
;
protein binding ( birA )
GO:0006350
;
transcription ( birA )
GO:0006355
;
regulation of transcription, DNA-dependent ( birA )
GO:0006464
;
protein modification process ( birA )
GO:0016564
;
transcription repressor activity ( birA )
GO:0016874
;
ligase activity ( birA )
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PID
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1790408
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EC number
(KEGG Pathway)
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6.3.4.15
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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BIRA_ECOLI
(
P06709
)
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- Protein name
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BirA bifunctional protein
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- Synonyms
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- Gene name
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Name=birA; Synonyms=bioR, dhbB; OrderedLocusNames=b3973;
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MMBR References
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Gene. 1985;35(3):321-31.
Nucleotide sequence of the birA gene encoding the biotin operon repressor and biotin holoenzyme synthetase functions of Escherichia coli.
Howard PK, Shaw J, Otsuka AJ.
(
3899863
)
|
J Bacteriol. 1980;143(2):789-800.
Use of bio-lac fusion strains to study regulation of biotin biosynthesis in Escherichia coli.
Barker DF, Campbell AM.
(
6782078
)
|
J Mol Biol. 1981;146(4):469-92.
Genetic and biochemical characterization of the birA gene and its product: evidence for a direct role of biotin holoenzyme synthetase in repression of the biotin operon in Escherichia coli.
Barker DF, Campbell AM.
(
6456358
)
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J Mol Biol. 1981;146(4):451-67.
The birA gene of Escherichia coli encodes a biotin holoenzyme synthetase.
Barker DF, Campbell AM.
(
7024555
)
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J Bacteriol. 1993;175(2):307-16.
Autogenous regulation of gene expression.
Maloy S, Stewart V.
(
8419283
)
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Mol Gen Genet. 1974;134(4):345-57.
Biochemical and genetic characterization of dehydrobiotin resistant mutants of Escherichia coli.
Pai CH.
(
4614081
)
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Biochemistry. 1996;35(6):1783-92.
Evidence for interdomain interaction in the Escherichia coli repressor of biotin biosynthesis from studies of an N-terminal domain deletion mutant.
Xu Y, Beckett D.
(
8639659
)
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J Bacteriol. 1980;142(3):1025-8.
Biotin regulatory (bir) mutations of Escherichia coli.
Campbell A, Chang R, Barker D, Ketner G.
(
6991489
)
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| Sequences |
Amino acid
FASTA format
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0001 MKDNTVPLKL IALLANGEFH SGEQLGETLG MSRAAINKHI QTLRDWGVDV FTVPGKGYSL PEPIQLLNAK
0071 QILGQLDGGS VAVLPVIDST NQYLLDRIGE LKSGDACIAE YQQAGRGRRG RKWFSPFGAN LYLSMFWRLE
0141 QGPAAAIGLS LVIGIVMAEV LRKLGADKVR VKWPNDLYLQ DRKLAGILVE LTGKTGDAAQ IVIGAGINMA
0211 MRRVEESVVN QGWITLQEAG INLDRNTLAA MLIRELRAAL ELFEQEGLAP YLSRWEKLDN FINRPVKLII
0281 GDKEIFGISR GIDKQGALLL EQDGIIKPWM GGEISLRSAE K
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ATGTAAGACA GAAAGTTGGT GAAAAATTTA
-070 ATGTCTGGCT TGAGCCTGAA GTCCGCTTTA TTGGTGCATC AGGTGAAGTG AGCGCAGTGG AGACAATTTC
0001 atgaaggata acaccgtgcc actgaaattg attgccctgt tagcgaacgg tgaatttcac tctggcgagc
0071 agttgggtga aacgctggga atgagccggg cggctattaa taaacacatt cagacactgc gtgactgggg
0141 cgttgatgtc tttaccgttc cgggtaaagg atacagcctg cctgagccta tccagttact taatgctaaa
0211 cagatattgg gtcagctgga tggcggtagt gtagccgtgc tgccagtgat tgactccacg aatcagtacc
0281 ttcttgatcg tatcggagag cttaaatcgg gcgatgcttg cattgcagaa taccagcagg ctggccgtgg
0351 tcgccggggt cggaaatggt tttcgccttt tggcgcaaac ttatatttgt cgatgttctg gcgtctggaa
0421 caaggcccgg cggcggcgat tggtttaagt ctggttatcg gtatcgtgat ggcggaagta ttacgcaagc
0491 tgggtgcaga taaagttcgt gttaaatggc ctaatgacct ctatctgcag gatcgcaagc tggcaggcat
0561 tctggtggag ctgactggca aaactggcga tgcggcgcaa atagtcattg gagccgggat caacatggca
0631 atgcgccgtg ttgaagagag tgtcgttaat caggggtgga tcacgctgca ggaagcgggg atcaatctcg
0701 atcgtaatac gttggcggcc atgctaatac gtgaattacg tgctgcgttg gaactcttcg aacaagaagg
0771 attggcacct tatctgtcgc gctgggaaaa gctggataat tttattaatc gcccagtgaa acttatcatt
0841 ggtgataaag aaatatttgg catttcacgc ggaatagaca aacagggggc tttattactt gagcaggatg
0911 gaataataaa accctggatg ggcggtgaaa tatccctgcg tagtgcagaa aaataaGAAA GGGGAGTATT
0981 CGCTCCCCTG CAAATTATTT GCGTAGTCTG ACCTCTTCTA CCGCATGATT AGCACTTTTC GTCAGGATTA
1051 AACTGGCGCG CTCACG
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