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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Mol Microbiol. 1995;16(1):45-55.
Genes acrA and acrB encode a stress-induced efflux system of Escherichia coli.
Ma D, Cook DN, Alberti M, Pon NG, Nikaido H, Hearst JE.
(
7651136
)
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|
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Deletion
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OCR41
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
acrB
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Related strains
|
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Strains Search
|
Search strains by gene name:
acrB
Search strains by all related name:
acrB acrE b0462 ECK0456 JW0451
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General information (Go to Linear View: )
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Gene Name
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acrB
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Alternative name
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acrE,b0462,ECK0456,JW0451
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Location, Length
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480,478 - 483,627
(
-
)
;
10.36
min
;
3150
(bp) ,
1049
(aa)
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Product
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multidrug efflux system protein
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Operon Name
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acrAB
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Note
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acridine efflux pump; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0042493 - response to drug
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Function
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transport; Drug/analog sensitivity
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Gene Ontology
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GO:0005215
;
transporter activity ( acrB )
GO:0005886
;
plasma membrane ( acrB )
GO:0005886
;
plasma membrane ( acrE )
GO:0006810
;
transport ( acrB )
GO:0007049
;
cell cycle ( acrE )
GO:0008565
;
protein transporter activity ( acrE )
GO:0009306
;
protein secretion ( acrE )
GO:0016020
;
membrane ( acrB )
GO:0016020
;
membrane ( acrE )
GO:0016021
;
integral to membrane ( acrB )
GO:0051301
;
cell division ( acrE )
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PID
|
1786667
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EC number
(KEGG Pathway)
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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ACRB_ECOLI
(
P31224
)
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- Protein name
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Acriflavine resistance protein B.
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- Synonyms
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- Gene name
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Name=acrB; Synonyms=acrE; OrderedLocusNames=b0462;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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acrB
(
homologous genes of other bacterias
)
acrE
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, Structural Basis Of The Multiple Binding Capacity Of The Acrb Multidrug Efflux Pump
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Acriflavine resistance protein B
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PSI-BLAST
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Acriflavine resistance protein B
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nr
(database updated :
2007.02.20
)
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BLAST
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Chain A, Asymmetric Structure Of Trimeric Acrb From Escherichia Coli
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
GLYCOSAMINOGLYCAN
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
AMIDATION
ATP_GTP_A
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MMBR References
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J Bacteriol. 1996;178:5803-5805
Evidence that TolC is required for functioning of the Mar/AcrAB efflux pump of Escherichia coli.
Fralick, J. A.
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J Bacteriol. 1993;175:6299-6313
Molecular cloning and characterization of acrA and acrE genes of Escherichia coli.
Ma, D., D. N. Cook, M. Alberti, N. G. Pon, H. Nikaido, and J. E. Hearst.
|
Mol Microbiol. 1995;16(1):45-55.
Genes acrA and acrB encode a stress-induced efflux system of Escherichia coli.
Ma D, Cook DN, Alberti M, Pon NG, Nikaido H, Hearst JE.
(
7651136
)
|
J Bacteriol. 1996;178:306-308
AcrAB efflux pump plays a major role in the antibiotic resistance phenotype of Escherichia coli multiple-antibiotic-resistance (Mar) mutants.
Okusu, H., D. Ma, and H. Nikaido.
|
J Bacteriol. 1997;179:6122-6126
Role of the acrAB locus in organic solvent tolerance mediated by expression of marA, soxS, or robA in Escherichia coli.
White, D. G., J. D. Goldman, B. Demple, and S. B. Levy.
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| Sequences |
Amino acid
FASTA format
|
0001 MPNFFIDRPI FAWVIAIIIM LAGGLAILKL PVAQYPTIAP PAVTISASYP GADAKTVQDT VTQVIEQNMN
0071 GIDNLMYMSS NSDSTGTVQI TLTFESGTDA DIAQVQVQNK LQLAMPLLPQ EVQQQGVSVE KSSSSFLMVV
0141 GVINTDGTMT QEDISDYVAA NMKDAISRTS GVGDVQLFGS QYAMRIWMNP NELNKFQLTP VDVITAIKAQ
0211 NAQVAAGQLG GTPPVKGQQL NASIIAQTRL TSTEEFGKIL LKVNQDGSRV LLRDVAKIEL GGENYDIIAE
0281 FNGQPASGLG IKLATGANAL DTAAAIRAEL AKMEPFFPSG LKIVYPYDTT PFVKISIHEV VKTLVEAIIL
0351 VFLVMYLFLQ NFRATLIPTI AVPVVLLGTF AVLAAFGFSI NTLTMFGMVL AIGLLVDDAI VVVENVERVM
0421 AEEGLPPKEA TRKSMGQIQG ALVGIAMVLS AVFVPMAFFG GSTGAIYRQF SITIVSAMAL SVLVALILTP
0491 ALCATMLKPI AKGDHGEGKK GFFGWFNRMF EKSTHHYTDS VGGILRSTGR YLVLYLIIVV GMAYLFVRLP
0561 SSFLPDEDQG VFMTMVQLPA GATQERTQKV LNEVTHYYLT KEKNNVESVF AVNGFGFAGR GQNTGIAFVS
0631 LKDWADRPGE ENKVEAITMR ATRAFSQIKD AMVFAFNLPA IVELGTATGF DFELIDQAGL GHEKLTQARN
0701 QLLAEAAKHP DMLTSVRPNG LEDTPQFKID IDQEKAQALG VSINDINTTL GAAWGGSYVN DFIDRGRVKK
0771 VYVMSEAKYR MLPDDIGDWY VRAADGQMVP FSAFSSSRWE YGSPRLERYN GLPSMEILGQ AAPGKSTGEA
0841 MELMEQLASK LPTGVGYDWT GMSYQERLSG NQAPSLYAIS LIVVFLCLAA LYESWSIPFS VMLVVPLGVI
0911 GALLAATFRG LTNDVYFQVG LLTTIGLSAK NAILIVEFAK DLMDKEGKGL IEATLDAVRM RLRPILMTSL
0981 AFILGVMPLV ISTGAGSGAQ NAVGTGVMGG MVTATVLAIF FVPVFFVVVR RRFSRKNEDI EHSHTVDHH
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gtaaaagcac aagaagttac cgctgataat
-070 aaccagcaag ccgcaagcgg tgctcagcct gaacagtcca agtcttaact taaacaggag ccgttaagac
0001 atgcctaatt tctttatcga tcgcccgatt tttgcgtggg tgatcgccat tatcatcatg ttggcagggg
0071 ggctggcgat cctcaaactg ccggtggcgc aatatcctac gattgcaccg ccggcagtaa cgatctccgc
0141 ctcctacccc ggcgctgatg cgaaaacagt gcaggacacg gtgacacagg ttatcgaaca gaatatgaac
0211 ggtatcgata acctgatgta catgtcctct aacagtgact ccacgggtac cgtgcagatc accctgacct
0281 ttgagtctgg tactgatgcg gatatcgcgc aggttcaggt gcagaacaaa ctgcagctgg cgatgccgtt
0351 gctgccgcaa gaagttcagc agcaaggggt gagcgttgag aaatcatcca gcagcttcct gatggttgtc
0421 ggcgttatca acaccgatgg caccatgacg caggaggata tctccgacta cgtggcggcg aatatgaaag
0491 atgccatcag ccgtacgtcg ggcgtgggtg atgttcagtt gttcggttca cagtacgcga tgcgtatctg
0561 gatgaacccg aatgagctga acaaattcca gctaacgccg gttgatgtca ttaccgccat caaagcgcag
0631 aacgcccagg ttgcggcggg tcagctcggt ggtacgccgc cggtgaaagg ccaacagctt aacgcctcta
0701 ttattgctca gacgcgtctg acctctactg aagagttcgg caaaatcctg ctgaaagtga atcaggatgg
0771 ttcccgcgtg ctgctgcgtg acgtcgcgaa gattgagctg ggtggtgaga actacgacat catcgcagag
0841 tttaacggcc aaccggcttc cggtctgggg atcaagctgg cgaccggtgc aaacgcgctg gataccgctg
0911 cggcaatccg tgctgaactg gcgaagatgg aaccgttctt cccgtcgggt ctgaaaattg tttacccata
0981 cgacaccacg ccgttcgtga aaatctctat tcacgaagtg gttaaaacgc tggtcgaagc gatcatcctc
1051 gtgttcctgg ttatgtatct gttcctgcag aacttccgcg cgacgttgat tccgaccatt gccgtaccgg
1121 tggtattgct cgggaccttt gccgtccttg ccgcctttgg cttctcgata aacacgctaa caatgttcgg
1191 gatggtgctc gccatcggcc tgttggtgga tgacgccatc gttgtggtag aaaacgttga gcgtgttatg
1261 gcggaagaag gtttgccgcc aaaagaagct acccgtaagt cgatggggca gattcagggc gctctggtcg
1331 gtatcgcgat ggtactgtcg gcggtattcg taccgatggc cttctttggc ggttctactg gtgctatcta
1401 tcgtcagttc tctattacca ttgtttcagc aatggcgctg tcggtactgg tggcgttgat cctgactcca
1471 gctctttgtg ccaccatgct gaaaccgatt gccaaaggcg atcacgggga aggtaaaaaa ggcttcttcg
1541 gctggtttaa ccgcatgttc gagaagagca cgcaccacta caccgacagc gtaggcggta ttctgcgcag
1611 tacggggcgt tacctggtgc tgtatctgat catcgtggtc ggcatggcct atctgttcgt gcgtctgcca
1681 agctccttct tgccagatga ggaccagggc gtgtttatga ccatggttca gctgccagca ggtgcaacgc
1751 aggaacgtac acagaaagtg ctcaatgagg taacgcatta ctatctgacc aaagaaaaga acaacgttga
1821 gtcggtgttc gccgttaacg gcttcggctt tgcgggacgt ggtcagaata ccggtattgc gttcgtttcc
1891 ttgaaggact gggccgatcg tccgggcgaa gaaaacaaag ttgaagcgat taccatgcgt gcaacacgcg
1961 ctttctcgca aatcaaagat gcgatggttt tcgcctttaa cctgcccgca atcgtggaac tgggtactgc
2031 aaccggcttt gactttgagc tgattgacca ggctggcctt ggtcacgaaa aactgactca ggcgcgtaac
2101 cagttgcttg cagaagcagc gaagcaccct gatatgttga ccagcgtacg tccaaacggt ctggaagata
2171 ccccgcagtt taagattgat atcgaccagg aaaaagcgca ggcgctgggt gtttctatca acgacattaa
2241 caccactctg ggcgctgcat ggggcggcag ctatgtgaac gactttatcg accgcggtcg tgtgaagaaa
2311 gtttatgtca tgtcagaagc gaaataccgt atgctgccgg atgatatcgg cgactggtat gttcgtgctg
2381 ctgatggtca gatggtgcca ttctcggcgt tctcctcttc tcgttgggag tacggttcgc cgcgtctgga
2451 acgttacaac ggcctgccat ccatggaaat cttaggccag gcggcaccgg gtaaaagtac cggtgaagca
2521 atggagctga tggaacaact ggcgagcaaa ctgcctaccg gtgttggcta tgactggacg gggatgtcct
2591 atcaggaacg tctctccggc aaccaggcac cttcactgta cgcgatttcg ttgattgtcg tgttcctgtg
2661 tctggcggcg ctgtacgaga gctggtcgat tccgttctcc gttatgctgg tcgttccgct gggggttatc
2731 ggtgcgttgc tggctgccac cttccgtggc ctgaccaatg acgtttactt ccaggtaggc ctgctcacaa
2801 ccattgggtt gtcggcgaag aacgcgatcc ttatcgtcga attcgccaaa gacttgatgg ataaagaagg
2871 taaaggtctg attgaagcga cgcttgatgc ggtgcggatg cgtttacgtc cgatcctgat gacctcgctg
2941 gcgtttatcc tcggcgttat gccgctggtt atcagtactg gtgctggttc cggcgcgcag aacgcagtag
3011 gtaccggtgt aatgggcggg atggtgaccg caacggtact ggcaatcttc ttcgttccgg tattctttgt
3081 ggtggttcgc cgccgcttta gccgcaagaa tgaagatatc gagcacagcc atactgtcga tcatcattga
3151 tacaacgtgt aatcactaag gccgcgtaag cggccttttt tatgcataac ctacgaacat taaggagtaa
3221 ttgaaccacc aactcaggat ctcatacgaa
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