MG1655
W3110
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Gene Report : leuS
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
J Bacteriol. 1971;108(2):742-50.
Isolation and partial characterization of temperature-sensitive Escherichia coli mutants with altered leucyl- and seryl-transfer ribonucleic acid synthetases.
Low B, Gates F, Goldstein T, Soll D. ( 4942762 )
     Deletion OCR46-11 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  leuS
Related strains
     Strains Search Search strains by gene name:  leuS   Search strains by all related name:  leuS b0642 ECK0635 JW0637

General information  (Go to Linear View:)
 Gene Name leuS  
 Alternative name b0642,ECK0635,JW0637  
 Location, Length 671,424 - 674,006 (  -  ) ;   14.47 min ; 2583 (bp) ,   860 (aa) Go to Linear View
 Product leucyl-tRNA synthetase  
 Operon Name leuS-lptE-holA-nadD-cobC  
 Note leucine tRNA synthetase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006418 - tRNA aminoacylation for protein translation  
 Function enzyme; Aminoacyl tRNA synthetases, tRNA modification  
 Gene Ontology GO:0000166 ; nucleotide binding ( leuS )
GO:0004812 ; aminoacyl-tRNA ligase activity ( leuS )
GO:0004823 ; leucine-tRNA ligase activity ( leuS )
GO:0005524 ; ATP binding ( leuS )
GO:0005737 ; cytoplasm ( leuS )
GO:0006412 ; translation ( leuS )
GO:0006418 ; tRNA aminoacylation for protein translation ( leuS )
GO:0006429 ; leucyl-tRNA aminoacylation ( leuS )
GO:0016874 ; ligase activity ( leuS )
 PID 1786861  
 EC number
  (KEGG Pathway)   		6.1.1.4  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) SYL_ECOLI ( P07813 ; P77110; P78292 )
    -  Protein name Leucyl-tRNA synthetase  
    -  Synonyms EC 6.1.1.4, Leucine--tRNA ligase, LeuRS  
    -  Gene name Name=leuS; OrderedLocusNames=b0642;  

 Linear View (Whole Mode)
View Location
   660.0  –  685.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP leuS (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Leucyl-Trna Synthetase From Thermus Thermophilus Complexed With A Sulphamoyl Analogue Of Leucyl-Adenylate  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Leucyl-tRNA synthetase  
         PSI-BLAST Leucyl-tRNA synthetase
    nr     (database updated : 2007.02.20 )
         BLAST leucyl-tRNA synthetase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AMIDATION    PROKAR_LIPOPROTEIN    AA_TRNA_LIGASE_I     

Other Cross-Reference
    COG COG0495J 
    EcoCyc leuS 

MMBR References
Nucleic Acids Res. 1987;15:10199-10210
Molecular cloning and nucleotide sequence of the gene for Escherichia coli leucyl-tRNA.
Hartlein, M., and D. Madern.
J Bacteriol. 1980;143(2):569-81.
Defective and plaque-forming lambda transducing bacteriophage carrying penicillin-binding protein-cell shape genes: genetic and physical mapping and identification of gene products from the lip-dacA-rodA-pbpA-leuS region of the Escherichia coli chromosome.
Spratt BG, Boyd A, Stoker N. ( 6451612 )

Sequences
Amino acid
FASTA format 
0001 MQEQYRPEEI ESKVQLHWDE KRTFEVTEDE SKEKYYCLSM LPYPSGRLHM GHVRNYTIGD VIARYQRMLG 
0071 KNVLQPIGWD AFGLPAEGAA VKNNTAPAPW TYDNIAYMKN QLKMLGFGYD WSRELATCTP EYYRWEQKFF 
0141 TELYKKGLVY KKTSAVNWCP NDQTVLANEQ VIDGCCWRCD TKVERKEIPQ WFIKITAYAD ELLNDLDKLD 
0211 HWPDTVKTMQ RNWIGRSEGV EITFNVNDYD NTLTVYTTRP DTFMGCTYLA VAAGHPLAQK AAENNPELAA 
0281 FIDECRNTKV AEAEMATMEK KGVDTGFKAV HPLTGEEIPV WAANFVLMEY GTGAVMAVPG HDQRDYEFAS 
0351 KYGLNIKPVI LAADGSEPDL SQQALTEKGV LFNSGEFNGL DHEAAFNAIA DKLTAMGVGE RKVNYRLRDW 
0421 GVSRQRYWGA PIPMVTLEDG TVMPTPDDQL PVILPEDVVM DGITSPIKAD PEWAKTTVNG MPALRETDTF 
0491 DTFMESSWYY ARYTCPQYKE GMLDSEAANY WLPVDIYIGG IEHAIMHLLY FRFFHKLMRD AGMVNSDEPA 
0561 KQLLCQGMVL ADAFYYVGEN GERNWVSPVD AIVERDEKGR IVKAKDAAGH ELVYTGMSKM SKSKNNGIDP 
0631 QVMVERYGAD TVRLFMMFAS PADMTLEWQE SGVEGANRFL KRVWKLVYEH TAKGDVAALN VDALTENQKA 
0701 LRRDVHKTIA KVTDDIGRRQ TFNTAIAAIM ELMNKLAKAP TDGEQDRALM QEALLAVVRM LNPFTPHICF 
0771 TLWQELKGEG DIDNAPWPVA DEKAMVEDST LVVVQVNGKV RAKITVPVDA TEEQVRERAG QEHLVAKYLD 
0841 GVTVRKVIYV PGKLLNLVVG
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             ggctgttttg acgcggacgt ttgggctatg 
-070 ctatgcggat ctgaaaaacc acctcaacgc tacatttgta gccgtattga aaacaggacc actggctgcc 
0001 atgcaagagc aataccgccc ggaagagata gaatccaaag tacagcttca ttgggatgag aagcgcacat 
0071 ttgaagtaac cgaagacgag agcaaagaga agtattactg cctgtctatg cttccctatc cttctggtcg 
0141 actacacatg ggccacgtac gtaactacac catcggtgac gtgatcgccc gctaccagcg tatgctgggc 
0211 aaaaacgtcc tgcagccgat cggctgggac gcgtttggtc tgcctgcgga aggcgcggcg gtgaaaaaca 
0281 acaccgctcc ggcaccgtgg acgtacgaca acatcgcgta tatgaaaaac cagctcaaaa tgctgggctt 
0351 tggttatgac tggagccgcg agctggcaac ctgtacgccg gaatactacc gttgggaaca gaaattcttc 
0421 accgagctgt ataaaaaagg cctggtatat aagaagactt ctgcggtcaa ctggtgcccg aacgaccaga 
0491 ccgtactggc gaacgaacaa gttatcgacg gctgctgctg gcgctgcgat accaaagttg aacgtaaaga 
0561 gatcccgcag tggtttatca aaatcactgc ttacgctgac gagctgctca acgatctgga taaactggat 
0631 cactggccag acaccgttaa aaccatgcag cgtaactgga tcggtcgttc cgaaggcgtg gagatcacct 
0701 tcaacgttaa cgactatgac aacacgctga ccgtttacac tacccgcccg gacaccttta tgggttgtac 
0771 ctacctggcg gtagctgcgg gtcatccgct ggcgcagaaa gcggcggaaa ataatcctga actggcggcc 
0841 tttattgacg aatgccgtaa caccaaagtt gccgaagctg aaatggcgac gatggagaaa aaaggcgtcg 
0911 atactggctt taaagcggtt cacccattaa cgggcgaaga aattcccgtt tgggcagcaa acttcgtatt 
0981 gatggagtac ggcacgggcg cagttatggc ggtaccgggg cacgaccagc gcgactacga gtttgcctct 
1051 aaatacggcc tgaacatcaa accggttatc ctggcagctg acggctctga gccagatctt tctcagcaag 
1121 ccctgactga aaaaggcgtg ctgttcaact ctggcgagtt caacggtctt gaccatgaag cggccttcaa 
1191 cgccatcgcc gataaactga ctgcgatggg cgttggcgag cgtaaagtga actaccgcct gcgcgactgg 
1261 ggtgtttccc gtcagcgtta ctggggcgcg ccgattccga tggtgacgct ggaagacggt accgtaatgc 
1331 cgaccccgga cgaccagctg ccggtgatcc tgccggaaga tgtggtaatg gacggcatta ccagcccgat 
1401 taaagcagat ccggagtggg cgaaaactac cgttaacggt atgccagcac tgcgtgaaac cgacactttc 
1471 gacaccttta tggagtcctc ctggtactat gcgcgctaca cttgcccgca gtacaaagaa ggtatgctgg 
1541 attccgaagc ggctaactac tggctgccgg tggatatcta cattggtggt attgaacacg ccattatgca 
1611 cctgctctac ttccgcttct tccacaaact gatgcgtgat gcaggcatgg tgaactctga cgaaccagcg 
1681 aaacagttgc tgtgtcaggg tatggtgctg gcagatgcct tctactatgt tggcgaaaac ggcgaacgta 
1751 actgggtttc cccggttgat gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag cgaaagatgc 
1821 ggcaggccat gaactggttt ataccggcat gagcaaaatg tccaagtcga agaacaacgg tatcgacccg 
1891 caggtgatgg ttgaacgtta cggcgcggac accgttcgtc tgtttatgat gtttgcttct ccggctgata 
1961 tgactctcga atggcaggaa tccggtgtgg aaggggctaa ccgcttcctg aaacgtgtct ggaaactggt 
2031 ttacgagcac acagcaaaag gtgatgttgc ggcactgaac gttgatgcgc tgactgaaaa tcagaaagcg 
2101 ctgcgtcgcg atgtgcataa aacgatcgct aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca 
2171 ccgcaattgc ggcgattatg gagctgatga acaaactggc gaaagcacca accgatggcg agcaggatcg 
2241 cgctctgatg caggaagcac tgctggccgt tgtccgtatg cttaacccgt tcaccccgca catctgcttc 
2311 acgctgtggc aggaactgaa aggcgaaggc gatatcgaca acgcgccgtg gccggttgct gacgaaaaag 
2381 cgatggtgga agactccacg ctggtcgtgg tgcaggttaa cggtaaagtc cgtgccaaaa tcaccgttcc 
2451 ggtggacgca acggaagaac aggttcgcga acgtgctggc caggaacatc tggtagcaaa atatcttgat 
2521 ggcgttactg tacgtaaagt gatttacgta ccaggtaaac tcctcaatct ggtcgttggc taagcgcggg 
2591 aggaagcgtg cgatatctgg caacattgtt gttatctctg gcggtgttaa tcaccgccgg gtgtggctgg 
2661 catctgcgtg ataccacgca ggt