MG1655
W3110
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Gene Report : kdpE
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion DE(pgl-grxA)502  ,  OCR48,49-8 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  kdpE
Related strains
     Strains Search Search strains by gene name:  kdpE   Search strains by all related name:  kdpE b0694 ECK0682 JW5096

General information  (Go to Linear View:)
 Gene Name kdpE  
 Alternative name b0694,ECK0682,JW5096  
 Location, Length 720,279 - 720,956 (  -  ) ;   15.52 min ; 678 (bp) ,   225 (aa) Go to Linear View
 Product DNA-binding response regulator in two-component regulatory system with KdpD  
 Operon Name kdpDE  
 Note regulator of kdp operon (transcriptional effector); GO_component: GO:0005737 - cytoplasm; GO_function: GO:0016563 - transcription activator activity; GO_process: GO:0006350 - transcription  
 Function regulator; Global regulatory functions  
 Gene Ontology GO:0000156 ; two-component response regulator activity ( kdpE )
GO:0000160 ; two-component signal transduction system (phosphorelay) ( kdpE )
GO:0003677 ; DNA binding ( kdpE )
GO:0005737 ; cytoplasm ( kdpE )
GO:0006350 ; transcription ( kdpE )
GO:0006355 ; regulation of transcription, DNA-dependent ( kdpE )
 PID 1786911  
 EC number
  (KEGG Pathway)
 
 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene kdpE ( EG10517 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) KDPE_ECOLI ( P21866 ; P75739; P76822 )
    -  Protein name KDP operon transcriptional regulatory protein kdpE.  
    -  Synonyms  
    -  Gene name Name=kdpE; OrderedLocusNames=b0694;  

 Linear View (Whole Mode)
View Location
   710.0  –  735.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP kdpE (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of The Response Regulatory Protein Prra From Mycobacterium Tuberculosis  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST KDP operon transcriptional regulatory protein kdpE  
         PSI-BLAST Probable transcriptional regulator ycf27
    nr     (database updated : 2007.02.20 )
         BLAST DNA-binding response regulator in two-component regulatory system with KdpD [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG0745TK 
    EcoCyc kdpE 

MMBR References
J Bacteriol. 1993;175:4528-4537
Regulation of kdp operon expression in Escherichia coli: evidence against turgor as signal for transcriptional control.
Asha, H., and J. Gowrishankar.
Mol Microbiol. 1993;7(1):109-16.
Signal transduction between the two regulatory components involved in the regulation of the kdpABC operon in Escherichia coli: phosphorylation-dependent functioning of the positive regulator, KdpE.
Nakashima K, Sugiura A, Kanamaru K, Mizuno T. ( 8437514 )
J. Biochem. (Tokyo) 1993;114:615-621
Functional reconstitution of the putative Escherichia coli osmosensor, KdpD, into liposomes.
Nakashima, K., A. Sugiura, and T. Mizuno.
J Bacteriol. 1992;174:2145-2151
The products of the kdpDE operon are required for expression of the Kdp ATPase of Escherichia coli.
Polarek, J. W., G. Williams, and W. Epstein.
Mol Microbiol. 1992;6(13):1769-76.
Clarification of the structural and functional features of the osmoregulated kdp operon of Escherichia coli.
Sugiura A, Nakashima K, Tanaka K, Mizuno T. ( 1630316 )
Eur J Biochem. 1993;217:1019-1026
Characterization of the KdpD protein, the sensor kinase of the K+-translocating Kdp system of Escherichia coli.
Voelkner, P., W. Puppe, and K. Altendorf.
J Bacteriol. 1992;174:2152-2159
KdpD and KdpE, proteins that control expression of the kdpABC operon, are members of the two-component sensor-effector class of regulators.
Walderhaug, M. O., J. W. Polarek, P. Voelkner, J. Daniel, J. E. Hesse, K. Altendorf, and W. Epstein.

Sequences
Amino acid
FASTA format
0001 MTNVLIVEDE QAIRRFLRTA LEGDGMRVFE AETLQRGLLE AATRKPDLII LDLGLPDGDG IEFIRDLRQW 
0071 SAVPVIVLSA RSEESDKIAA LDAGADDYLS KPFGIGELQA RLRVALRRHS ATTAPDPLVK FSDVTVDLAA 
0141 RVIHRGEEEV HLTPIEFRLL AVLLNNAGKV LTQRQLLNQV WGPNAVEHSH YLRIYMGHLR QKLEQDPARP 
0211 RHFITETGIG YRFML

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             gcactattac cgcgttcaac cgaccggaag 
-070 gtggtgcctg ttttcgtgtt acacttcccc agcaaactgc ccctgaactt gaagaatttc atgaggatat 
0001 gtgacaaacg ttctgattgt tgaagatgaa caggctattc gtcgctttct gcgcacggcg ctggagggcg 
0071 acgggatgcg cgtctttgag gccgaaacgc tgcaacgcgg cttgctggaa gcggcaaccc gtaagccaga 
0141 tttgattatt ctcgatctcg gcctgcccga tggtgatggg attgagttta tccgcgacct gcgccagtgg 
0211 agcgcggtgc cggtgattgt gctttccgca cgcagcgaag agagcgacaa aatcgccgcg ctggatgccg 
0281 gagcggatga ttatctgagt aagccgtttg gcattggcga attgcaggcc cgtctgcgcg tcgcattacg 
0351 ccgccactct gccaccaccg cgcccgatcc gctggtaaaa ttttccgatg ttaccgtcga tttagccgcc 
0421 cgcgtgattc accggggtga ggaagaggtg catctcacac caattgagtt ccgcctgctg gcggtgctgc 
0491 tcaacaatgc cggaaaagta ctcacccagc gccagctcct taaccaggtg tgggggccaa acgcggtcga 
0561 acacagtcac tatttgcgta tttatatggg acatctgcga caaaaactgg aacaggatcc cgcccgccca 
0631 cgccatttca ttactgaaac cggtattggc tatcggttta tgctttgaat attaatttta atacagcctg 
0701 ccttttatta attaaagccg taataataaa tacggctttt tatcttaaac aacacacaaa aataacaatt 
0771 caatattt