|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
J Bacteriol. 1997;179(11):3773-82.
Escherichia coli endonuclease VIII: cloning, sequencing, and overexpression of the nei structural gene and characterization of nei and nei nth mutants.
Jiang D, Hatahet Z, Blaisdell JO, Melamede RJ, Wallace SS.
(
9171429
)
|
|
|
Deletion
|
DE(pgl-grxA)502
,
DE(kdpABC-gltA)219
,
OCR48,49-6
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
nei
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
nei
Search strains by all related name:
nei b0714 ECK0703 JW0704
|
General information (Go to Linear View: )
|
|
Gene Name
|
nei
|
|
Alternative name
|
b0714,ECK0703,JW0704
|
|
Location, Length
|
745,158 - 745,949
(
+
)
;
16.06
min
;
792
(bp) ,
263
(aa)
|
|
Product
|
endonuclease VIII/5-formyluracil/5-hydroxymethyluracil DNA glycosylase
|
|
Operon Name
|
ybgIJKL-nei
|
|
Note
|
endonuclease VIII and DNA N-glycosylase with an AP lyase activity; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006281 - DNA repair
|
|
Function
|
enzyme; DNA - replication, repair,restriction/modification
|
|
Gene Ontology
|
GO:0000703
;
oxidized pyrimidine base lesion DNA N-glycosylase activity ( nei )
GO:0003676
;
nucleic acid binding ( nei )
GO:0003677
;
DNA binding ( nei )
GO:0003684
;
damaged DNA binding ( nei )
GO:0003824
;
catalytic activity ( nei )
GO:0003906
;
DNA-(apurinic or apyrimidinic site) lyase activity ( nei )
GO:0006281
;
DNA repair ( nei )
GO:0006284
;
base-excision repair ( nei )
GO:0006289
;
nucleotide-excision repair ( nei )
GO:0006974
;
response to DNA damage stimulus ( nei )
GO:0008152
;
metabolic process ( nei )
GO:0008270
;
zinc ion binding ( nei )
GO:0008534
;
oxidized purine base lesion DNA N-glycosylase activity ( nei )
GO:0016787
;
hydrolase activity ( nei )
GO:0016798
;
hydrolase activity, acting on glycosyl bonds ( nei )
GO:0016829
;
lyase activity ( nei )
GO:0046872
;
metal ion binding ( nei )
|
|
PID
|
1786932
|
EC number
(KEGG Pathway)
|
3.2.-.-
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
nei
(
EG13237
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
END8_ECOLI
(
P50465
)
|
|
- Protein name
|
Endonuclease VIII
|
|
- Synonyms
|
EC 3.2.-.-
|
|
- Gene name
|
Name=nei; OrderedLocusNames=b0714;
|
|
MMBR References
|
Nucleic Acids Res. 1998;26:932-941
Multiply damaged sites in DNA: interactions with Escherichia coli endonucleases III and VIII.
Harrison, L., Z. Hatahet, A. A. Purmal, and S. S. Wallace.
|
J Bacteriol. 1997;179:3773-3782
Escherichia coli endonuclease VIII: cloning, sequencing, and overexpression of the nei structural gene and characterization of nei and nei nth mutants.
Jiang, D., Z. Hatahet, J. O. Blaisdell, R. J. Melamede, and S. S. Wallace.
|
J Biol Chem. 1997;272:32230-32239
Characterization of Escherichia coli endonuclease VIII.
Jiang, D., Z. Hatahet, R. J. Melamede, Y. W. Kow, and S. S. Wallace.
|
Biochemistry. 1994;33(5):1255-64.
Isolation and characterization of endonuclease VIII from Escherichia coli.
Melamede RJ, Hatahet Z, Kow YW, Ide H, Wallace SS.
(
8110759
)
|
J Bacteriol. 1997;179:3783-3785
Characterization of endonuclease III (nth) and endonuclease VIII (nei) mutants of Escherichia coli K-12.
Saito, Y., F. Uraki, S. Nakajima, A. Asaeda, K. Ono, K. Kubo, and K. Yamamoto.
|
| Sequences |
Amino acid
FASTA format
|
0001 MPEGPEIRRA ADNLEAAIKG KPLTDVWFAF PQLKPYQSQL IGQHVTHVET RGKALLTHFS NDLTLYSHNQ
0071 LYGVWRVVDT GEEPQTTRVL RVKLQTADKT ILLYSASDIE MLTPEQLTTH PFLQRVGPDV LDPNLTPEVV
0141 KERLLSPRFR NRQFAGLLLD QAFLAGLGNY LRVEILWQVG LTGNHKAKDL NAAQLDALAH ALLEIPRFSY
0211 ATRGQVDENK HHGALFRFKV FHRDGEPCER CGSIIEKTTL SSRPFYWCPG CQH
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CACTGGCGTT CGCCCGCCGA CTACGCTCTG
-070 CATTTGCCGA AAAGGGGATT GTTGTCGCAG CATAACCCCG ATTAATAAAG AATGAAAAAA GGATATCACC
0001 atgcctgaag gcccggagat ccgccgtgca gcggataacc tggaggcggc gatcaaaggc aaaccactaa
0071 ctgatgtctg gtttgccttc ccgcagttaa aaccttatca atcacaactt atcggtcaac acgttaccca
0141 tgtggaaacg cgtggtaagg cgttgttaac tcatttttcc aacgacttaa cgctctacag ccataatcag
0211 ctttacggcg tctggcgcgt ggttgatacc ggcgaagagc cgcagaccac gcgagtattg cgggtaaaac
0281 tgcaaacggc tgacaaaacc attctgcttt atagcgcctc ggatattgag atgttgaccc cggaacaact
0351 gaccacgcat ccgtttttac aacgcgttgg tcccgatgtg ctggatccga atctgacgcc ggaggtggtg
0421 aaagaacgat tattgtcgcc gcgctttcgt aaccgtcagt ttgctggatt actgctcgat caggcgtttc
0491 tggctgggct tggcaattat ttgcgggtgg agatcctctg gcaggttggg ttgactggaa atcataaagc
0561 gaaagatctc aatgcggcgc aactggatgc actcgcacac gcgttactgg agattcctcg attttcctac
0631 gctacgcggg ggcaggtgga tgagaataag catcatgggg cgctgtttcg ctttaaggtt tttcatcgag
0701 atggcgaacc gtgcgaacgt tgtggcagca tcattgagaa aaccacgctg tcatctcgcc cgttttactg
0771 gtgccctggc tgccagcact agGCCGACCG CTTCGGCGCA TAGGTTGAAA TAAACCGCGC AATGGCAGGC
0841 CCTGTCAGCA AAATACTGAA CAGGCGTAGG GTTTGCATCG CCATAATGAG CG
|
