|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Proc Natl Acad Sci U S A. 1990;87(1):360-3.
Mutations in the bglY gene increase the frequency of spontaneous deletions in Escherichia coli K-12.
Lejeune P, Danchin A.
(
2404280
)
|
|
|
Deletion
|
15
(LD)
,
DE(pgl-grxA)502
,
DE(galETK-chlA)
,
15+dps
(LD)
,
OCR48,49-5
(D)
,
OCR48,50-8 (LD 15D::Cm)
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
bioD
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
bioD
Search strains by all related name:
bioD b0778 ECK0767 JW0761
|
General information (Go to Linear View: )
|
|
Gene Name
|
bioD
|
|
Alternative name
|
b0778,ECK0767,JW0761
|
|
Location, Length
|
811,493 - 812,170
(
+
)
;
17.49
min
;
678
(bp) ,
225
(aa)
|
|
Product
|
dethiobiotin synthetase
|
|
Operon Name
|
bioBFCD
|
|
Note
|
GO_process: GO:0009102 - biotin biosynthetic process
|
|
Function
|
enzyme; Biosynthesis of cofactors, carriers: Biotin
|
|
Gene Ontology
|
GO:0000166
;
nucleotide binding ( bioD )
GO:0000287
;
magnesium ion binding ( bioD )
GO:0004141
;
dethiobiotin synthase activity ( bioD )
GO:0005524
;
ATP binding ( bioD )
GO:0005737
;
cytoplasm ( bioD )
GO:0009102
;
biotin biosynthetic process ( bioD )
GO:0009236
;
cobalamin biosynthetic process ( bioD )
GO:0016874
;
ligase activity ( bioD )
GO:0042242
;
cobyrinic acid a,c-diamide synthase activity ( bioD )
|
|
PID
|
1786995
|
EC number
(KEGG Pathway)
|
6.3.3.3
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
bioD
(
EG10120
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
BIOD_ECOLI
(
P13000
)
|
|
- Protein name
|
Dethiobiotin synthetase
|
|
- Synonyms
|
EC 6.3.3.3, Dethiobiotin synthase, DTB synthetase, DTBS
|
|
- Gene name
|
Name=bioD; OrderedLocusNames=b0778;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
bioD
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain , Dethiobiotin Synthetase (E.C.6.3.3.3)
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Dethiobiotin synthetase
|
|
PSI-BLAST
|
CTP synthase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
dethiobiotin synthetase [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
AMIDATION
ATP_GTP_A
|
|
MMBR References
|
Proc Natl Acad Sci U S A. 1968;61(3):956-62.
A deletion analysis of prophage lambda and adjacent genetic regions.
Adhya S, Cleary P, Campbell A.
(
5246560
)
|
Mol Microbiol. 1996;19(3):645-7.
To be free or not: the fate of pimelate in Bacillus sphaericus and in Escherichia coli.
Lemoine Y, Wach A, Jeltsch JM.
(
8830257
)
|
J. Microbiol. Serol. 1969;35:505-521
A genetical and biochemical study of chlorate-resistant mutants of Salmonella typhimurium. Antonie Leeuwenhoek.
Stouthamer, A. H.
|
Gene. 1982;19(1):93-103.
A physical map of the Escherichia coli bio operon.
Szybalski EH, Szybalski W.
(
6292053
)
|
Biochemistry. 1997;36(16):4751-60.
Active site mutants of Escherichia coli dethiobiotin synthetase: effects of mutations on enzyme catalytic and structural properties.
Yang G, Sandalova T, Lohman K, Lindqvist Y, Rendina AR.
(
9125495
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSKRYFVTGT DTEVGKTVAS CALLQAAKAA GYRTAGYKPV ASGSEKTPEG LRNSDALALQ RNSSLQLDYA
0071 TVNPYTFAEP TSPHIISAQE GRPIESLVMS AGLRALEQQA DWVLVEGAGG WFTPLSDTFT FADWVTQEQL
0141 PVILVVGVKL GCINHAMLTA QVIQHAGLTL AGWVANDVTP PGKRHAEYMT TLTRMIPAPL LGEIPWLAEN
0211 PENAATGKYI NLALL
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ATATTAACGC GTTCGCAGTT GCAGCGATTG
-070 CAACTGGCCT GGCCGCAACA GCAGGGGCGA TATCCTCTGA CGTATCATCT TTTTTTGGGA GTGATTGCTC
0001 gtgagtaaac gttattttgt caccggaacg gataccgaag tggggaaaac tgtcgccagt tgtgcacttt
0071 tacaagccgc aaaggcagca ggctaccgga cggcaggtta taaaccggtc gcctctggca gcgaaaagac
0141 cccggaaggt ttacgcaata gcgacgcgct ggcgttacag cgcaacagca gcctgcagct ggattacgca
0211 acagtaaatc cttacacctt cgcagaaccc acttcgccgc acatcatcag cgcgcaagag ggcagaccga
0281 tagaatcatt ggtaatgagc gccggattac gcgcgcttga acaacaggct gactgggtgt tagtggaagg
0351 tgctggcggc tggtttacgc cgctttctga cactttcact tttgcagatt gggtaacaca ggaacaactg
0421 ccggtgatac tggtagttgg tgtgaaactc ggctgtatta atcacgcgat gttgactgca caggtaatac
0491 aacacgccgg actgactctg gcgggttggg tggcgaacga tgttacgcct ccgggaaaac gtcacgctga
0561 atatatgacc acgctcaccc gcatgattcc cgcgccgctg ctgggagaga tcccctggct tgcagaaaat
0631 ccagaaaatg cggcaaccgg aaagtacata aaccttgcct tgttgtagCC ATTCTGTATT TGGTTAAATT
0701 GCGAGCGAGA TCGCGTCTTC GATTGACTGC AATTTAACCA ATTAAATTCT AAAATAATCA CGAAAAAAAT
0771 TTTACTTC
|
