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PEC Original Annotations
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Essentiality
|
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Class
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non-essential
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References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
|
OCR56
(D)
,
OCR56-8
(D)
|
|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
helD
|
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Related strains
|
|
Strains Search
|
Search strains by gene name:
helD
Search strains by all related name:
helD b0962 ECK0953 JW0945 srjB
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General information (Go to Linear View: )
|
|
Gene Name
|
helD
|
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Alternative name
|
b0962,ECK0953,JW0945,srjB
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|
Location, Length
|
1,023,694 - 1,025,748
(
+
)
;
22.06
min
;
2055
(bp) ,
684
(aa)
|
|
Product
|
DNA helicase IV
|
|
Operon Name
|
helD
|
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Note
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GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006261 - DNA-dependent DNA replication
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Function
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enzyme; DNA - replication, repair,restriction/modification
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|
Gene Ontology
|
GO:0000155
;
two-component sensor activity ( helD )
GO:0000166
;
nucleotide binding ( helD )
GO:0003677
;
DNA binding ( helD )
GO:0004003
;
ATP-dependent DNA helicase activity ( helD )
GO:0004386
;
helicase activity ( helD )
GO:0005515
;
protein binding ( helD )
GO:0005524
;
ATP binding ( helD )
GO:0006281
;
DNA repair ( helD )
GO:0007165
;
signal transduction ( helD )
GO:0016020
;
membrane ( helD )
GO:0016787
;
hydrolase activity ( helD )
|
|
PID
|
1787196
|
EC number
(KEGG Pathway)
|
3.6.1.-
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
helD
(
EG10426
)
|
|
Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
HELD_ECOLI
(
P15038
;
P77623
)
|
|
- Protein name
|
Helicase IV
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- Synonyms
|
EC 3.6.1.-, 75 kDa helicase
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- Gene name
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Name=helD; OrderedLocusNames=b0962;
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Homology Analysis
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BLAST
|
|
Bacteria
|
|
GTOP
|
helD
(
homologous genes of other bacterias
)
|
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PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain , Structure Of Dna Helicase
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
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BLAST
|
Helicase IV
|
|
PSI-BLAST
|
UvrABC system protein B
|
|
nr
(database updated :
2007.02.20
)
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BLAST
|
DNA helicase IV [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
|
|
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PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
AMIDATION
ATP_GTP_A
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MMBR References
|
Genetics 1995;140:27-45
Suppression of recJ exonuclease mutants of Escherichia coli by alterations in DNA helicases II (uvrD) and IV (helD).
Lovett, S. T., and V. A. Sutera.
|
J Bacteriol. 1995;177:1326-1335
DNA helicases in recombination and repair: construction of a Delta uvrD Delta helD Delta recQ mutant deficient in recombination and repair.
Mendonca, V. M., H. D. Klepin, and S. W. Matson.
|
J Bacteriol. 1993;175:4641-4651
Double helicase II (uvrD)-helicase IV(helD) deletion mutants are defective in the recombination pathways of Escherichia coli.
Mendonca, V. M., K. Kaiser-Rogers, and S. W. Matson.
|
Genetics 1995;141:443-452
Genetic analysis of delta helD and delta uvrD mutations in combination with other genes in the RecF recombination pathway in Escherichia coli: suppression of a ruvB mutation by a uvrD deletion.
Mendonca, V. M., and S. W. Matson.
|
J Biol Chem. 1989;264:8297-8303
The molecular cloning of the gene encoding the Escherichia coli 75-kDa helicase and the determination of its nucleotide sequence and genetic map position.
Wood, E. R., and S. W. Matson.
|
| Sequences |
Amino acid
FASTA format
|
0001 MELKATTLGK RLAQHPYDRA VILNAGIKVS GDRHEYLIPF NQLLAIHCKR GLVWGELEFV LPDEKVVRLH
0071 GTEWGETQRF YHHLDAHWRR WSGEMSEIAS GVLRQQLDLI ATRTGENKWL TREQTSGVQQ QIRQALSALP
0141 LPVNRLEEFD NCREAWRKCQ AWLKDIESAR LQHNQAYTEA MLTEYADFFR QVESSPLNPA QARAVVNGEH
0211 SLLVLAGAGS GKTSVLVARA GWLLARGEAS PEQILLLAFG RKAAEEMDER IRERLHTEDI TARTFHALAL
0281 HIIQQGSKKV PIVSKLENDT AARHELFIAE WRKQCSEKKA QAKGWRQWLT EEMQWSVPEG NFWDDEKLQR
0351 RLASRLDRWV SLMRMHGGAQ AEMIASAPEE IRDLFSKRIK LMAPLLKAWK GALKAENAVD FSGLIHQAIV
0421 ILEKGRFISP WKHILVDEFQ DISPQRAALL AALRKQNSQT TLFAVGDDWQ AIYRFSGAQM SLTTAFHENF
0491 GEGERCDLDT TYRFNSRIGE VANRFIQQNP GQLKKPLNSL TNGDKKAVTL LDESQLDALL DKLSGYAKPE
0561 ERILILARYH HMRPASLEKA ATRWPKLQID FMTIHASKGQ QADYVIIVGL QEGSDGFPAA ARESIMEEAL
0631 LPPVEDFPDA EERRLMYVAL TRARHRVWAL FNKENPSPFV EILKNLDVPV ARKP
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 TTCAGTAATG GGCGGCAATT GTAACGTTTT
-070 TTTGGGCTGG AGCACGTTTT CTCTGACGGT TACACTGATA AGAAATAATT TCGTGTGGAT CTACAGAGTC
0001 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg gtgatcctca
0071 atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc aatcaattac tggcgattca
0141 ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta ctgccggacg aaaaagtggt gcgtctgcac
0211 ggcaccgaat ggggcgagac gcagcgtttt taccatcatc ttgatgctca ctggcggcgg tggagtggcg
0281 agatgagcga aattgcgtct ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggggaaaa
0351 taaatggctg acgcgtgagc aaacctctgg cgtgcagcaa caaatccgcc aggctttgtc ggcgttgccg
0421 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag gcctggttga
0491 aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc atgcttaccg agtatgcgga
0561 ttttttccgc caggtcgagt cttcaccgct gaatccggcg caggcccggg cagtcgttaa tggcgagcat
0631 tctctgttag tgctggcagg tgcaggaagc ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc
0701 tggcgcgtgg tgaagcgtcc cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat
0771 ggacgagcgg attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg
0841 catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc gctgcccgtc
0911 atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg caggcgaagg gctggcggca
0981 atggctgacg gaagaaatgc agtggtcagt gccagaaggt aacttctggg atgatgaaaa attacagcgt
1051 cgccttgcct ctcgcctcga tcgctgggta agtctgatgc ggatgcacgg tggtgcacag gcagaaatga
1121 ttgccagtgc acccgaagag attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa
1191 agcctggaaa ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg
1261 attctggaga aaggtcgctt tatcagcccg tggaagcata ttctggttga tgaatttcag gatatctcgc
1331 cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg acgttgttcg ctgttggtga
1401 tgactggcag gcgatttacc gattcagcgg tgcgcaaatg tcgctcacca ccgctttcca tgaaaacttt
1471 ggtgaaggcg aacgctgtga tttagacacg acttaccgtt ttaacagtcg tatcggtgag gtggcaaacc
1541 ggtttattca gcagaaccca ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc
1611 cgtcacgtta ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa
1681 gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg gcaacacgct
1751 ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa caggcggatt acgtcatcat
1821 cgttggcttg caggagggaa gtgatggttt tccggctgcg gcgcgggagt cgattatgga agaggcgcta
1891 ctgccaccgg ttgaggattt cccggacgct gaagaacggc ggttaatgta cgtggcgctg acccgggcac
1961 gccatcgggt atgggcactg tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga
2031 tgtgccggtg gcgagaaaac cgtaaGAAAC AGGTGGCGTT TGCCACCTGT GCAATATTAC TTCAGACGGT
2101 CCGCGAGATA ACGCTGATAA TCGGGGATCA GAATATCGAC CGCGTCGTTG AAATG
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