Strain name	Gene type	Reference (RRC ID)
01stepAW	W3110, Δ(1049227-1097311)	
02stepAW	01stepAW, Δ(3109247-3133480)	
03stepAW	02stepAW, Δ(4239495-4255870)	
04stepAW	03stepAW, Δ(1198444-1225484)	
05stepAW	04stepAW, Δ(1630054-1640180), Δ(1641770-1649361)	
06stepAW	05stepAW, Δ(518367-533048), Δ(535841-550551), Δ(564277-608454)	
07stepAW	06stepAW, Δ(3486428-3496908), Δ(3536156-3549686), Δ(3556478-3576238)	
08stepBW	07stepAW, Δ(2984498-3032266)	
09stepAW	08stepBW, Δ(244301-253746), Δ(262300-387867), Δ(389475-404039)	
10stepCW	09stepAW, Δ(3697305-3709526), Δ(3762909-3772816), Δ(3774088-3785257)	
11stepBW	10stepCW, Δ(1963664-1992215), Δ(1995801-2027348), Δ(2032588-2043515), Δ(2068289-2081066), Δ(2103532-2115203)	
12stepEW	11stepBW, Δ(2754815-2788618), Δ(2794312-2800028), Δ(2824452-2829398)	
13stepDW	12stepEW, Δ(3361081-3370891)	
14stepAγW	13stepDW, Δ(2145403-2186994), Δ(2199808-2228989), Δ(2255035-2262631)	
15stepEγW	14stepAγW, Δ(3076224-3078055)	
16stepBγW	15stepEγW, Δ(4501438-4514770), Δ(4523114-4604874)	
17stepBγW	16stepBγW, Δ(729157-739929)	
18stepBγW	17stepBγW, Δ(4414768-4428387)	
19stepGW	18stepBγW, Δ(4003005-4021346)	
20stepCW	19stepGW, Δ(832889-848426), Δ(867975-883810), Δ(891335-909716)	
21stepGW	20stepCW, Δ(3164770-3172081)	
22stepEW	21stepGW, Δ(675440-689710)	
23stepAW	22stepEW, Δ(2422504-2433237), Δ(2471991-2481621)	
24stepAW	23stepAW, Δ(4170563-4186908)	
25stepBW	24stepAW, Δ(2385401-2394610)	
26stepAW	25stepBW, Δ(3411500-3421220)	
27stepAW	26stepAW, Δ(2909410-2909709)	
MGF-01W	27stepAW, Δ(167401-173315)	
MGF-01WS	MGF-01W, ilvG::(functional ilvG from E.coliB), pyrE::(functional pyrE from E.coliB)	
MGF-01K-S	MGF-01WS, 【Δ(4239495-4255870)::wild, this region was restored】	
MGF-02W	MGF-01W, Δ(640669-659192), Δ(2921194-2943638), Δ(65857-78797), Δ(4128291-4141015), Δ(1853580-1864142), Δ(3794688-3803458), Δ(3224890-3240400), Δ(4055372-4061465), Δ(1875288-1888499), Δ(2876630-2876783), Δ(2599858-2613439)	
MGF-02WS	MGF-02W, ilvG::(functional ilvG from E.coliB), pyrE::(functional pyrE from E.coli B)	
MGF-02WSΔ234::SC	MGF-02WS, Δ(2921194-2943638)::(sacB, cat)	
W3110S	W3110, ilVG::(functional ilvG from E.coliB), pyr::(functional pyrE from E.coli B) 	
DGF-327WΔ234::SC	MGF-02WSΔ234::SC, Δ(1651508-1657863), Δ(2494688-2513688), Δ(3871777-3914528), Δ(4447062-4453719), Δ(1592568-1599800), Δ(3746428-3751980), Δ(1296003-1308864), Δ(1098128-1117068), Δ(1522387-1543782), Δ(15445-16903), Δ(19646-20508), Δ(1359517-1368530), Δ(2519718-2523278), Δ(2909410-2909709), Δ(3183787-3188521), Δ(2289724-2293603), Δ(2411459-2412234), Δ(2091353-2091607), Δ(3987068-3989225), Δ(2091353-2091607), Δ(3987068-3989225), Δ(3918367-3923869), Δ(3932710-3938551)	
DGF-298WΔ100::revΔ234::SC	DGF-327WΔ234::SC, Δ(2361574-2382929), Δ(1130992-1142564), Δ(2836910-2855462), Δ(3730114-3742029), Δ(3058023-3068464), Δ(3258359-3269672), Δ(1607823-1624231), Δ(1582557-1592251), Δ(1396605-1520045), Δ(4281498-4291254), Δ(4298072-4366613), Δ(747145-753217), Δ(3918091-3969123), 【Δ(2909410-2909709)::wild, this region was restored】