遺伝子変異株 - 詳細

ME No. ME9395
菌株名 AQ8022
性別 F-
組み込みプラスミド
自律複製プラスミド
プロファージ
遺伝子型 trpA9605 his-29 proB(or C) ilv metB1 thyA deoB(or C) recJ284::dTn10(Tc-s)
親株(供与菌)
親株(受容菌) AQ3362
変異導入方法 OTHER METHODSBochner selection
最終選別マーカー recJ284::dTn10(Tc-s)
選別マーカー Tc-(Bochner selection)
入手源
ゲノムマップ
文献 Kogoma T, Hong X, Cadwell GW, Barnard KG, Asai T.
Requirement of homologous recombination functions for viability of the Escherichia coli cell that lacks RNase HI and exonuclease V activities.
Biochimie  (1993)  75(1-2)   89-99  
[PubMed ID = 8389213]  [RRC文献情報
培地条件 Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
その他備考 Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
分譲依頼
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