pAP27 plasmid and primers to amplify the donor DNA for SDSA mediated knock-in construct. The crRNA and tracer RNA for recognizing the myosin heavy chain locus are also included. These are useful for benchmark experiments of KI to tag the endogenous protein using fluorescence proteins. Cost of this set is equivalent with 7 clones.
Backbone vector
pUC119
Antibiotics Resistance
Amp
Reference
Category
Plasmid and the biotinylated primers for SDSA mediated knock-in
