ABC transporter a1a gene was genome edited with CRISPR/Cas9. 8 nucleotides in coding sequence were removed to make frame-shift mutation. Hetero zygote (male and female) has no effect. But Homo zygote of female does not spawn.
The RECIPIENT shall refer the following publication in any PUBLICATIONS. (Name of the Publication: Futamata R, Kinoshita M, Ogiwara K, Kioka N, Ueda K. Cholesterol accumulation in ovarian follicles causes ovulation defects in Abca1a Japanese medaka (Oryzias latipes). Heliyon, 10.1016/j.heliyon.2023.e13291)
