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11.1.1 Periodic acid-Schiff (PAS) staining of the kidney†
Emiko Mochiduki
Tomohiro Harada
Nagoya University
11.1.1.1 Procedure†
- Fix the tissues with Bouin?s fixative.
- Prepare paraffin sections by a conventional method.
- Put deparaffinized sections in 0.5% periodic acid solution in 5 min.
- Rinse sections in two changes of distilled water (DW) for 1 min. each.
- Put sections in cold Schiff?s reagent for 5 min.
- Put sections in three changes of H2SO3 solution for three min. each.
- Rinse sections in running tap water for ten min. Regulate the time with checking the degree of staining.
- Put sections in hematoxylin for 3-4 min.
- Rinse sections in running tap water for ten min. Regulate the time with checking the degree of staining.
- Dehydrate and mount sections by a conventional method.
11.1.1.2 Notices†