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H.1.1 Periodic acid-Schiff (PAS) staining of the kidney
Emiko Mochiduki
Tomohiro Harada
Nagoya University
H.1.1.1 Procedure
- Fix the tissues with Bouin”Ēs fixative.
- Prepare paraffin sections by a conventional method.
- Put deparaffinized sections in 0.5% periodic acid solution in 5 min.
- Rinse sections in two changes of distilled water (DW) for 1 min. each.
- Put sections in cold Schiff”Ēs reagent for 5 min.
- Put sections in three changes of H2SO3 solution for three min. each.
- Rinse sections in running tap water for ten min. Regulate the time with checking the degree of staining.
- Put sections in hematoxylin for 3-4 min.
- Rinse sections in running tap water for ten min. Regulate the time with checking the degree of staining.
- Dehydrate and mount sections by a conventional method.
H.1.1.2 Notices
- All procedures are processed at room temperature.
- Bouin”Ēs fixative:
Saturated picric acid solution 15 ml
Formaldehyde solution 5 ml
Acetic acid 1 ml
Mix the above solutions before use.
- Cold Schiff:
Stock a commercially available reagent at 4”ī, and keep it at room temperature for 30 min. before use. The reagent losing the odor cannot be used.
- H2SO3 solution:
10%NaHSO3 6 ml
1N HCl 5 ml
DW 100 ml
- Staining of the kidney:
A photo of PAS-stained section is attached.
The basement membrane of the renal tubules, glomerulus and Bouman”Ēs capsule, and the apical surface of the proximal tubule segment is stained with purple color. d/c, distal or collecting tubule segment; g, glomerulus; p, proximal tubule segment; l, lymphoid tissues.